UM
In vitro immunomodulatory effects of human milk oligosaccharides on murine macrophage RAW264.7 cells
Zhang, Weiyue1; Yan, Jingyu2; Wu, Lehao1; Yu, Yang1; Ye, Richard D.1,3; Zhang, Yan1; Liang, Xinmiao2
2019-03
Source PublicationCARBOHYDRATE POLYMERS
ISSN0144-8617
Volume207Pages:230-238
AbstractHuman milk plays an important role in the child's immune system. The human milk oligosaccharides (HMOs) may affect breast-fed infants both locally and systemically. In the present study, HMOs were separated, characterized and investigated for the immunomodulatory effects on RAW264.7 macrophages and its underlying molecular mechanisms. Our results revealed that HMO-7, one of the neutral HMOs fractions can significantly induce the production of nitric oxide (NO) and PGE2 via up-regulating nitric oxide synthase (iNOS) and cyclooxygenase 2 (COX-2) expression. Additionally, HMO-7 was found to stimulate the release of ROS, TNF-alpha and cytokines including IL-1 beta, IL-2, IL-6 and IL-10 in RAW264.7 macrophages. Further study showed that macrophage activated by HMO-7 involved in nuclear factor (NF)-kappa B and mitogen-activated protein kinase (MAPK) signaling pathways. Our study provides additional evidence that HMOs are the functional components in human milk and that HMOs may have the potential application in healthcare industry.
KeywordHuman milk oligosaccharides Immunomodulator Nitric oxide Cytokine NF-kappa B MAPKs
DOI10.1016/j.carbpol.2018.11.039
URLView the original
Indexed BySCI
Language英语
WOS Research AreaChemistry ; Polymer Science
WOS SubjectChemistry, Applied ; Chemistry, Organic ; Polymer Science
WOS IDWOS:000454537500025
PublisherELSEVIER SCI LTD
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Cited Times [WOS]:4   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
Affiliation1.Shanghai Jiao Tong Univ, Sch Pharm, Shanghai 200240, Peoples R China;
2.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China;
3.Univ Macau, Inst Chinese Med Sci, Macau, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Weiyue,Yan, Jingyu,Wu, Lehao,et al. In vitro immunomodulatory effects of human milk oligosaccharides on murine macrophage RAW264.7 cells[J]. CARBOHYDRATE POLYMERS,2019,207:230-238.
APA Zhang, Weiyue.,Yan, Jingyu.,Wu, Lehao.,Yu, Yang.,Ye, Richard D..,...&Liang, Xinmiao.(2019).In vitro immunomodulatory effects of human milk oligosaccharides on murine macrophage RAW264.7 cells.CARBOHYDRATE POLYMERS,207,230-238.
MLA Zhang, Weiyue,et al."In vitro immunomodulatory effects of human milk oligosaccharides on murine macrophage RAW264.7 cells".CARBOHYDRATE POLYMERS 207(2019):230-238.
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