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Anti-neuroinflammatory effects of SLOH in A beta-induced BV-2 microglial cells and 3xTg-AD mice involve the inhibition of GSK-3 beta
Wu, Xiaoli; Kosaraju, Jayasankar; Tam, Kin Yip
2018-11-20
Source PublicationNEUROSCIENCE LETTERS
ISSN0304-3940
Volume687Pages:207-215
AbstractNeuroinflammation has been observed in post-mortem Alzheimer's disease (AD) brains which could be due to A beta interacting with microglia and astrocytes. SLOH, a carbazole-based fluorophore, was shown to bind to A beta peptides. Herein, we investigated the anti-neuroinflammatory effects of SLOH using a BV-2 microglial cell model and a triple transgenic AD (3xTg-AD) mouse model. BV-2 cells were incubated with A beta in the presence of SLOH for 24 h. The levels of pro-inflammatory and anti-inflammatory cytokines were determined. Moreover, 3xTg-AD mice were administrated with SLOH (2 mg kg(-1)) for one month. The mice were then sacrificed and the brains were used to assess the levels of pro-inflammatory, anti-inflammatory cytokines and the activation of ionized calcium-binding adapter molecule 1 (Iba1). BV-2 cell studies suggested that SLOH reduced the production and mRNA levels of pro-inflammatory cytokines TNF-alpha, IL-1 beta, COX-2, iNOS, and increased IL-10. Animal study confirmed that SLOH reduced the production of pro-inflammatory cytokines and increased the level of anti-inflammatory cytokine. Moreover, SLOH inhibited the activity of GSK-3 beta. In 3xTg-AD mouse model, SLOH treatment significantly decreased the number of Ibal-positive cells in mouse brains. Our results demonstrated that SLOH significantly attenuated the neuroinflammation through down-regulating the activity of GSK-3 beta.
KeywordSLOH BV-2 cells 3xTg-AD mice Neuroinflammation GSK-3 beta
DOI10.1016/j.neulet.2018.09.056
URLView the original
Indexed BySCI
Language英语
WOS Research AreaNeurosciences & Neurology
WOS SubjectNeurosciences
WOS IDWOS:000452346000034
PublisherELSEVIER IRELAND LTD
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Cited Times [WOS]:2   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionFaculty of Health Sciences
AffiliationUniv Macau, Fac Hlth Sci, Taipa, Macau, Peoples R China
First Author AffilicationUniversity of Macau
Recommended Citation
GB/T 7714
Wu, Xiaoli,Kosaraju, Jayasankar,Tam, Kin Yip. Anti-neuroinflammatory effects of SLOH in A beta-induced BV-2 microglial cells and 3xTg-AD mice involve the inhibition of GSK-3 beta[J]. NEUROSCIENCE LETTERS,2018,687:207-215.
APA Wu, Xiaoli,Kosaraju, Jayasankar,&Tam, Kin Yip.(2018).Anti-neuroinflammatory effects of SLOH in A beta-induced BV-2 microglial cells and 3xTg-AD mice involve the inhibition of GSK-3 beta.NEUROSCIENCE LETTERS,687,207-215.
MLA Wu, Xiaoli,et al."Anti-neuroinflammatory effects of SLOH in A beta-induced BV-2 microglial cells and 3xTg-AD mice involve the inhibition of GSK-3 beta".NEUROSCIENCE LETTERS 687(2018):207-215.
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