UM  > 中華醫藥研究院
Simultaneous quantification six active compounds in rat plasma by UPLC-MS/MS and its application to a pharmacokinetic study of Pien-Tze-Huang
Xu, Wen1,2; Zhang, Yiping2; Zhou, Caijie3; Tai, Yanni1; Zhang, Xiaoqing1; Liu, Jie1; Sha, Mei1; Huang, Mingqing1,2; Zhu, Yanlin1; Peng, Jun4; Lu, Jin-Jian5
2017-09
Source PublicationJOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
ISSN1570-0232
Volume1061Pages:314-321
Abstract

Pien-Tze-Huang (PZH) is a popular traditional Chinese medicine (TCM) formula in China, but its pharmacokinetics has not been investigated yet. To better study the pharmacokinetic behaviors of PZH, an optimal ultra-performance liquid chromatography with triple quadrupole mass spectrometry (UPLC-MS/MS) method was developed for rapid quantification of six compounds (notoginsenoside R1, ginsenosides Re, Rgl, Rb1, Rd, and muscone) in rat plasma after oral administration of PZH. All analytes were extracted by protein precipitation with acetonitrile and separated on a Waters Acquity Cortecs C18 column within 3.9 min, and detected by multiple-reaction monitoring in positive ion mode. This proposed method exhibited good linearity (r >= 0.9932) with a lower quantification limits of 0.558-1.566 ng/mL for all analytes. The infra- and inter-day precisions were within 8.24%, and the accuracy was within -10.05 to 9.87% for each analyte. The extraction recovery for each analyte ranged from 80.02 to 96.12%. This UPLC-MS/MS method was successfully applied to the pharmacokinetic study for PZH in rats.

KeywordPian-tze-huang Uplc-ms/ms Ginsenosides Muscone Pharmacokinetics
DOI10.1016/j.jchromb.2017.07.033
URLView the original
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS IDWOS:000411542000043
PublisherELSEVIER SCIENCE BV
The Source to ArticleWOS
Fulltext Access
Citation statistics
Cited Times [WOS]:7   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Affiliation1.College of Pharmacy, Fujian Key Laboratory of Chinese Materia Medica, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China
2.Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, China
3.Shenzhen Key Laboratory of ENT, Institute of ENT, Longgang ENT Hospital, Shenzhen 518172, China
4.Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China
5.State Key Laboratory for Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, China
Recommended Citation
GB/T 7714
Xu, Wen,Zhang, Yiping,Zhou, Caijie,et al. Simultaneous quantification six active compounds in rat plasma by UPLC-MS/MS and its application to a pharmacokinetic study of Pien-Tze-Huang[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2017,1061:314-321.
APA Xu, Wen.,Zhang, Yiping.,Zhou, Caijie.,Tai, Yanni.,Zhang, Xiaoqing.,...&Lu, Jin-Jian.(2017).Simultaneous quantification six active compounds in rat plasma by UPLC-MS/MS and its application to a pharmacokinetic study of Pien-Tze-Huang.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,1061,314-321.
MLA Xu, Wen,et al."Simultaneous quantification six active compounds in rat plasma by UPLC-MS/MS and its application to a pharmacokinetic study of Pien-Tze-Huang".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 1061(2017):314-321.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Xu, Wen]'s Articles
[Zhang, Yiping]'s Articles
[Zhou, Caijie]'s Articles
Baidu academic
Similar articles in Baidu academic
[Xu, Wen]'s Articles
[Zhang, Yiping]'s Articles
[Zhou, Caijie]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Xu, Wen]'s Articles
[Zhang, Yiping]'s Articles
[Zhou, Caijie]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.