| Hierarchical identification of bioactive components in a medicinal herb by preparative high-performance liquid chromatography and selective knock-out strategy | |
Qu, Cheng; Wang, Lin-Yan; Lin, Hang; Shang, Er-Xin; Tang, Yu-Ping; Yue, Shi-Jun; Jin, Yi; Tao, Wei-Wei; Li, Shao-Ping ; Hua, Yong-Qing; Liu, Pei; Su, Shu-Lan; Zhou, Huiping; Qian, Da-Wei; Duan, Jin-Ao
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| 2017-02-20 | |
| Source Publication | JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
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| ISSN | 0731-7085 |
| Volume | 135Pages:206-216 |
| Abstract | A novel and generally applicable approach was established to hierarchically identify the bioactive components of a medicinal herb by preparative high-performance liquid chromatography (prep-HPLC) and a selective knock-out strategy. In this study, the targeted components of an herbal medicine were separated and knocked out using prep-HPLC. Subsequently, the contributions of the different target components to the overall effect of the medicinal herb were comparatively evaluated and differentiated by a heat map and a 3D score plot. This approach was successfully applied to investigate the bioactive constituents of safflower. The contributions of 11 components to the overall effect of safflower were as follows: anhydrosafflor yellow B(10)>6-hydroxykaempferol 3,6-di-O-beta-D-glucoside (8 > hydroxysafflor yellow A (3)> kaempferol 3-O-beta-rutinoside (11)> 6-hydroxykaempferol 3-O-beta-rutinoside (9)> 6-hydroxykaempferol 3,6-di-O-beta-D-glucoside-7-O-beta-D-glucuronide (4)> 6-hydroxyapigenin 6-O-D-glucoside-7-O-beta-D-glucuronide (6) > cytidine (1)> 6-hydroxykaempferol 3-O-beta-rutinoside-6-O-beta-D-glucoside (7)> 6-hydroxykaempferol 3,6,7-tri-O-beta-D-glucoside (5) > adenosine (2). These results demonstrate that quinochalcone C-glycosides (3 and 10) and some flavonoid glycosides containing C7-OH (such as 8, 9 and 11) made a greater contribution to the overall effect of safflower than the other components that were knocked out. The results provided an important reference for improving quality control and further development of safflower products. And this approach should also be useful for investigating the bioactive constituents of other medicinal herbs. (C) 2016 Elsevier B.V. All rights reserved. |
| Keyword | Safflower Herb Contribution Component High-performance liquid chromatography Knock-out |
| DOI | 10.1016/j.jpba.2016.12.027 |
| URL | View the original |
| Indexed By | SCI |
| Language | 英语 |
| WOS Research Area | Chemistry ; Pharmacology & Pharmacy |
| WOS Subject | Chemistry, Analytical ; Pharmacology & Pharmacy |
| WOS ID | WOS:000392905200023 |
| Publisher | ELSEVIER SCIENCE BV |
| The Source to Article | WOS |
| Fulltext Access | |
| Citation statistics | |
| Document Type | Journal article |
| Collection | Institute of Chinese Medical Sciences |
| Recommended Citation GB/T 7714 | Qu, Cheng,Wang, Lin-Yan,Lin, Hang,et al. Hierarchical identification of bioactive components in a medicinal herb by preparative high-performance liquid chromatography and selective knock-out strategy[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,2017,135:206-216. |
| APA | Qu, Cheng.,Wang, Lin-Yan.,Lin, Hang.,Shang, Er-Xin.,Tang, Yu-Ping.,...&Duan, Jin-Ao.(2017).Hierarchical identification of bioactive components in a medicinal herb by preparative high-performance liquid chromatography and selective knock-out strategy.JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,135,206-216. |
| MLA | Qu, Cheng,et al."Hierarchical identification of bioactive components in a medicinal herb by preparative high-performance liquid chromatography and selective knock-out strategy".JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS 135(2017):206-216. |
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