UM  > 個人在本單位外知識產出
Mechanism of the cardioprotection of rhEPO pretreatment on suppressing the inflammatory response in ischemia-reperfusion
Liu,Xiaoming1; Xie,Weiying1; Liu,Pichu2; Duan,Manlin1; Jia,Zhen1; Li,Wei1; Xu,Jianguo1
2006-04-04
Source PublicationLife Sciences
ISSN00243205
Volume78Issue:19Pages:2255-2264
AbstractErythropoietin (EPO), originally known for its role in stimulation of erythropoiesis, has recently been shown to have a dramatic protective effect in animal models of myocardial ischemia-reperfusion (I-R) injury. However, the precise mechanisms remain unclear. We tried to study the anti-inflammatory properties of recombinant human erythropoietin (rhEPO) using an in vivo myocardial I-R rat model, which was established by 30 min ligation of left descending coronary and 3 h reperfusion. rhEPO or saline solution was intraperitoneally injected 24 h before I-R insult. The infarct size was measured by triphenyltetrazolium chloride (TTC)-Evans blue technique. Myeloperoxidase (MPO) activity and tissue neutrophil infiltration were studied. Ultrastructural organizations were observed and semiquantitatively evaluated. Tumor necrosis-alpha (TNF-α), interleukin-6 (IL-6), and IL-10 concentrations of left ventricle were analyzed by enzyme-linked immunosorbance assays; intercellular adhesion molecule-1 (ICAM-1) by reverse-transcription polymerase chain reaction; and nuclear factor-kappa B (NF-κB) and activator protein 1 (AP-1) by electrophoretic mobility shift assay, respectively. We found that a single bolus injection of 5000 units/kg of rhEPO 24 h before insult remarkably reduced infarct size and neutrophil infiltration. It greatly attenuated I-R-induced NF-κB and AP-1 activation with decreased TNF-α, IL-6, and ICAM-1 production, but enhanced IL-10 production. In conclusion, the cardioprotection of EPO may be due in part to the suppression of the inflammatory response via down-regulation of NF-κB and AP-1 induced by I-R. IL-10 was also suggested to play a protective role through another independent mechanism involved in cardioprotection of rhEPO. © 2005 Elsevier Inc. All rights reserved.
KeywordAP-1 Erythropoietin IL-10 Ischemia-reperfusion NF-κB
DOI10.1016/j.lfs.2005.09.053
URLView the original
Language英语
Fulltext Access
Citation statistics
Document TypeJournal article
CollectionPersonal research not belonging to the institution
Affiliation1.Department of Anesthesiology,Jinling Hospital,Nanjing University,,China
2.Department of Surgery,Jinling Hospital,Nanjing University,,China
Recommended Citation
GB/T 7714
Liu,Xiaoming,Xie,Weiying,Liu,Pichu,et al. Mechanism of the cardioprotection of rhEPO pretreatment on suppressing the inflammatory response in ischemia-reperfusion[J]. Life Sciences,2006,78(19):2255-2264.
APA Liu,Xiaoming.,Xie,Weiying.,Liu,Pichu.,Duan,Manlin.,Jia,Zhen.,...&Xu,Jianguo.(2006).Mechanism of the cardioprotection of rhEPO pretreatment on suppressing the inflammatory response in ischemia-reperfusion.Life Sciences,78(19),2255-2264.
MLA Liu,Xiaoming,et al."Mechanism of the cardioprotection of rhEPO pretreatment on suppressing the inflammatory response in ischemia-reperfusion".Life Sciences 78.19(2006):2255-2264.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Liu,Xiaoming]'s Articles
[Xie,Weiying]'s Articles
[Liu,Pichu]'s Articles
Baidu academic
Similar articles in Baidu academic
[Liu,Xiaoming]'s Articles
[Xie,Weiying]'s Articles
[Liu,Pichu]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Liu,Xiaoming]'s Articles
[Xie,Weiying]'s Articles
[Liu,Pichu]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.