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Anti-inflammatory activities of Sigesbeckia glabrescens Makino: combined in vitro and in silico investigations
Zhangfeng Zhong1,4; Qianru Zhang1,5; Hongxun Tao1; Wei Sang1; Liao Cui4; Wenan Qiang6,7; Wai San Cheang1; Yuanjia Hu1; Hua Yu1,2,3; Yitao Wang1
2019-09-23
Source PublicationCHINESE MEDICINE
ISSN1749-8546
Volume14Issue:1
Abstract

Background: Sigesbeckia glabrescens Makino (SG) is one of the important plant origins of Sigesbeckiae herba and has been widely used for the treatment of chronic infammatory diseases in China. However, the underlying anti-infammatory mechanism of SG is rarely investigated and reported. There are more than 40 kinds of chemical constituents in SG, but the action of the bioactive compounds of SG is still unclear. Therefore, we aimed to systemically investigate the mechanisms behind the anti-infammatory properties of SG by combining in vitro and in silico investigations. Methods: Cytotoxicity was measured using the 3-[4,5-dimethyl-2-thiazolyl]-2,5-diphenyltetrazolium bromide (MTT) and lactate dehydrogenase (LDH) assays. Nitric oxide (NO) release was detected using the Griess assay. The secretion of pro-infammatory cytokines and the expression of relevant proteins were assessed using ELISA kits and Western blots, respectively. Molecular docking was performed and scored using AutoDock via a comparison with the molecular docking of N-acetyl-d-glucosamine (NAG). Results: In lipopolysaccharides (LPS)-stimulated macrophages, SG signifcantly inhibited NO, MCP-1, and IL-6 secretion; iNOS expression; and NF-κB activation but did not signifcantly afect MAPK signalling (p38, ERK, and JNK). Moreover, the results from the molecular docking prediction suggested that over 10 compounds in SG could likely target TLR4, p105, and p65. Conclusions: These fndings suggest that the anti-infammatory efects of SG are highly related to the inactivation of NF-κB. Moreover, this study provides a novel approach to investigate the efects of herbal medicine using combined in vitro and in silico investigations.

KeywordSigesbeckia Glabrescens Makino Infammation Lipopolysaccharide Docking Tlr4 Nf-κb
DOI10.1186/s13020-019-0260-y
Indexed BySCI
Language英语
WOS Research AreaIntegrative & Complementary Medicine ; Pharmacology & Pharmacy
WOS SubjectIntegrative & Complementary Medicine ; Pharmacology & Pharmacy
WOS IDWOS:000487483800003
PublisherBMC, CAMPUS, 4 CRINAN ST, LONDON N1 9XW, ENGLAND
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Cited Times [WOS]:3   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Corresponding AuthorYuanjia Hu; Hua Yu
Affiliation1.Institute of Chinese Medical Sciences, State Key Laboratory of Quality Research in Chinese Medicine, University of Macau, Macao, China
2.HKBU Shenzhen Research Center, Shenzhen, China
3.School of Chinese Medicine, Hong Kong Baptist University, Kowloon Tong, China
4.Guangdong Key Laboratory for Research and Development of Natural Drugs, Guangdong Medical University, Zhanjiang, China
5.School of Pharmacy, Zunyi Medical University, Zunyi, China
6.Center for Developmental Therapeutics, Chemistry of Life Processes Institute, Northwestern University, Evanston, USA
7.Devision of Reproductive Science in Medicine, Department of Obstetrics and Gynecology, , Feinberg School of Medicine, Northwestern University, Chicago, USA
First Author AffilicationInstitute of Chinese Medical Sciences
Corresponding Author AffilicationInstitute of Chinese Medical Sciences
Recommended Citation
GB/T 7714
Zhangfeng Zhong,Qianru Zhang,Hongxun Tao,et al. Anti-inflammatory activities of Sigesbeckia glabrescens Makino: combined in vitro and in silico investigations[J]. CHINESE MEDICINE,2019,14(1).
APA Zhangfeng Zhong.,Qianru Zhang.,Hongxun Tao.,Wei Sang.,Liao Cui.,...&Yitao Wang.(2019).Anti-inflammatory activities of Sigesbeckia glabrescens Makino: combined in vitro and in silico investigations.CHINESE MEDICINE,14(1).
MLA Zhangfeng Zhong,et al."Anti-inflammatory activities of Sigesbeckia glabrescens Makino: combined in vitro and in silico investigations".CHINESE MEDICINE 14.1(2019).
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