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Metabolic Profiling of Saponin-Rich Ophiopogon japonicus Roots Based on(1)H NMR and HPTLC Platforms1
Yanhui Ge1,2; Xiaojia Chen3; Dejan Gođevac1,4; PaulaC.P.Bueno1; LuisF.Salomé Abarca1; Young Pyo Jang5; Mei Wang6,7; Young Hae Choi1,5
2019-08
Source PublicationPLANTA MEDICA
ISSN0032-0943
Volume85Issue:11/12Pages:917-924
Abstract

Ideally, metabolomics should deal with all the metabolites that are found within cells and biological systems. The most common technologies for metabolomics include mass spectrometry, and in most cases, hyphenated to chromatographic separations (liquid chromatography- or gas chromatography-mass spectrometry) and nuclear magnetic resonance spectroscopy. However, limitations such as low sensitivity and highly congested spectra in nuclear magnetic resonance spectroscopy and relatively low signal reproducibility in mass spectrometry impede the progression of these techniques from being universal metabolomics tools. These disadvantages are more notorious in studies of certain plant secondary metabolites, such as saponins, which are difficult to analyse, but have a great biological importance in organisms. In this study, high-performance thin-layer chromatography was used as a supplementary tool for metabolomics. A method consisting of coupling (1) H nuclear magnetic resonance spectroscopy and high-performance thin-layer chromatography was applied to distinguish between Ophiopogon japonicus roots that were collected from two growth locations and were of different ages. The results allowed the root samples from the two growth locations to be clearly distinguished. The difficulties encountered in the identification of the marker compounds by (1) H nuclear magnetic resonance spectroscopy was overcome using high-performance thin-layer chromatography to separate and isolate the compounds. The saponins, ophiojaponin C or ophiopogonin D, were found to be marker metabolites in the root samples and proved to be greatly influenced by plant growth location, but barely by age variation. The procedure used in this study is fully described with the purpose of making a valuable contribution to the quality control of saponin-rich herbal drugs using high-performance thin-layer chromatography as a supplementary analytical tool for metabolomics research.

KeywordMetabolic Profiling Ophiopogon Japonicus Asparagaceae Saponin Nmr Hptlc Age Growth Location
DOI10.1055/a-0947-5797
Indexed BySCI
Language英语
WOS Research AreaPlant Sciences ; Pharmacology & Pharmacy ; Integrative & Complementary Medicine
WOS SubjectPlant Sciences ; Chemistry, Medicinal ; Integrative & Complementary Medicine ; Pharmacology & Pharmacy
WOS IDWOS:000479140800009
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Cited Times [WOS]:0   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Affiliation1.Natural Products Laboratory, Institute of Biology, LeidenUniversity, Leiden, The Netherlands
2.School of Pharmacy, Xiʼan Jiaotong University, Xiʼan, China
3.State Key Laboratory of Quality Research in Chinese Medi-cine, Institute of Chinese Medical Sciences, University ofMacau, Macao, China
4.Institute of Chemistry, Technology and Metallurgy, Na-tional Institute, University of Belgrade, Belgrade, Serbia
5.College of Pharmacy, Kyung Hee University, Seoul 02447,Republic of Korea
6.LU-European Center for Chinese Medicine and NaturalCompounds, Leiden, The Netherlands
7.SU Biomedicine, Leiden, The Netherlands
Recommended Citation
GB/T 7714
Yanhui Ge,Xiaojia Chen,Dejan Gođevac,et al. Metabolic Profiling of Saponin-Rich Ophiopogon japonicus Roots Based on(1)H NMR and HPTLC Platforms1[J]. PLANTA MEDICA,2019,85(11/12):917-924.
APA Yanhui Ge.,Xiaojia Chen.,Dejan Gođevac.,PaulaC.P.Bueno.,LuisF.Salomé Abarca.,...&Young Hae Choi.(2019).Metabolic Profiling of Saponin-Rich Ophiopogon japonicus Roots Based on(1)H NMR and HPTLC Platforms1.PLANTA MEDICA,85(11/12),917-924.
MLA Yanhui Ge,et al."Metabolic Profiling of Saponin-Rich Ophiopogon japonicus Roots Based on(1)H NMR and HPTLC Platforms1".PLANTA MEDICA 85.11/12(2019):917-924.
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