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Rapid and visual detection of aflatoxin B1 in foodstuffs using aptamer/G-quadruplex DNAzyme probe with low background noise
Wang, Lumin1; Zhu, Fawei1; Chen, Miao1; Zhu, Yuqiu1; Xiao, Jianbo3; Yang, Hua1; Chen, Xiaoqing1,2
2019-01-15
Conference Name3rd International Symposium on Phytochemicals in Medicine and Food (ISPMF)
Source PublicationFOOD CHEMISTRY
Volume271
Pages581-587
Conference DateAUG 25-30, 2018
Conference PlaceKunming, PEOPLES R CHINA
Publication PlaceTHE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND
PublisherELSEVIER SCI LTD
Abstract

Contamination of foods by aflatoxin B1 (AFB1) is a common serious problem. To improve the efficiency of AFB1 detection, this study aims to develop a sensitive aptasensor for detection of AFB1 in food samples based on the catalytic effect of aptamer/G-quadruplex DNAzyme probe. The resulting reassembly of this probe in the presence of hemin and K+ catalyzes the generation of fluorescent 2,3-diaminophenazine (DAP) from o-phenylenediamine (OPD). Interestingly, we first found that the high background induced by the superfluous hemin can be effectively suppressed with the aid of sequential adsorption and magnetic separation by magnetic oxidized multiwall carbon nanotubes (Fe3O4@oMWCNTs). This aptasensor exhibits a high sensitivity toward AFB1 with a detection limit of 0.02 ng/mL. The assay also shows higher selectivity for AFB1 compared to other reported agents and can be employed to detect AFB1 in foodstuffs, which might find broad practical applications in other food contaminants determination.

KeywordFluorescence Aptamer Dnazyme Magnetic Oxidized Multiwall Carbon Nanotubes Aflatoxin B1 Foodstuffs
DOI10.1016/j.foodchem.2018.08.007
URLView the original
Indexed BySCI ; CPCI
Language英语
WOS Research AreaChemistry ; Food Science & Technology ; Nutrition & Dietetics
WOS SubjectChemistry, Applied ; Food Science & Technology ; Nutrition & Dietetics
WOS IDWOS:000444967800074
The Source to ArticleWOS
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Cited Times [WOS]:13   [WOS Record]     [Related Records in WOS]
Document TypeConference paper
CollectionInstitute of Chinese Medical Sciences
Affiliation1.College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, Hunan, China
2.Hunan Provincial Key Laboratory of Efficient and Clean Utilization of Manganese Resources, Central South University, Changsha 410083, Hunan, China
3.Institute of Chinese Medical Sciences, State Key Laboratory of Quality Research in Chinese Medicine, University of Macau, Taipa, Macau, China
Recommended Citation
GB/T 7714
Wang, Lumin,Zhu, Fawei,Chen, Miao,et al. Rapid and visual detection of aflatoxin B1 in foodstuffs using aptamer/G-quadruplex DNAzyme probe with low background noise[C]. THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND:ELSEVIER SCI LTD,2019:581-587.
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