Ganoderiol A-enriched extract suppresses migration and adhesion via inhibiting FAK-SRC-paxillin cascade pathway in MDA-MB-231 cells

doi:https://doi.org/10.1371/journal.pone.0076620

UM > Institute of Chinese Medical Sciences

	Ganoderiol A-enriched extract suppresses migration and adhesion via inhibiting FAK-SRC-paxillin cascade pathway in MDA-MB-231 cells
	Wu, GS 1; Song, YL 1; Yin, ZQ 2; Guo, JJ 1; Wang, SP1 ; Zhao, WW 1; Chen, XP 1; Zhang, QW1 ; Lu, JJ1 ; Wang, YT1
	2013
Source Publication	ACTA PHARMACOLOGICA SINICA
ISSN	1671-4083
Volume	34 Pages:32-32
Abstract	Cell adhesion, migration and invasion are critical steps for carcinogenesis and cancer metastasis. Ganoderma lucidum, also called Lingzhi in China, is a traditional Chinese medicine, which exhibits anti-proliferation, anti-inflammation and anti-metastasis properties. Herein, GAEE, G. lucidum extract mainly contains ganoderiol A (GA), dihydrogenated GA and GA isomer, was shown to inhibit the abilities of adhesion and migration, while have a slight influence on that of invasion in highly metastatic breast cancer MDA-MB-231 cells at non-toxic doses. Further investigation revealed that GAEE decreased the active forms of focal adhesion kinase (FAK) and disrupted the interaction between FAK and SRC, which lead to deactivating of paxillin. Moreover, GAEE treatment downregulated the expressions of RhoA, Rac1, and Cdc42, and decreased the interaction between neural Wiskott-Aldrich Syndrome protein (N-WASP) and Cdc42, which impair cell migration and actin assembly. To our knowledge, this is the first report to show that G.lucidum triterpenoids could suppress cell migration and adhesion through FAK-SRC-paxillin signaling pathway. Our study also suggests that GAEE may be a potential agent for treatment of breast cancer.
DOI	https://doi.org/10.1371/journal.pone.0076620
Indexed By	SCI
Language	英语
WOS Research Area	Chemistry ; Pharmacology & Pharmacy
WOS Subject	Chemistry, Multidisciplinary ; Pharmacology & Pharmacy
WOS ID	WOS:000322051500142
Fulltext Access	View Full-Text via DOI View Full-Text via Web of Science
Citation statistics	Cited Times [WOS]:1 [WOS Record] [Related Records in WOS]
Document Type	Journal article
Collection	Institute of Chinese Medical Sciences
Corresponding Author	Wang, YT
Affiliation	1.University of Macau 2.China Pharmaceut Univ, Dept Nat Med Chem, Nanjing, Jiangsu, Peoples R China
First Author Affilication	University of Macau
Corresponding Author Affilication	University of Macau
Recommended Citation GB/T 7714	Wu, GS,Song, YL,Yin, ZQ,et al. Ganoderiol A-enriched extract suppresses migration and adhesion via inhibiting FAK-SRC-paxillin cascade pathway in MDA-MB-231 cells[J]. ACTA PHARMACOLOGICA SINICA,2013,34:32-32.
APA	Wu, GS.,Song, YL.,Yin, ZQ.,Guo, JJ.,Wang, SP.,...&Wang, YT.(2013).Ganoderiol A-enriched extract suppresses migration and adhesion via inhibiting FAK-SRC-paxillin cascade pathway in MDA-MB-231 cells.ACTA PHARMACOLOGICA SINICA,34,32-32.
MLA	Wu, GS,et al."Ganoderiol A-enriched extract suppresses migration and adhesion via inhibiting FAK-SRC-paxillin cascade pathway in MDA-MB-231 cells".ACTA PHARMACOLOGICA SINICA 34(2013):32-32.

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