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A combination of Pueraria lobata and Silybum marianum protects against alcoholic liver disease in mice
Feng Ruibing1; Chen Jie-Hua2; Liu Cong-Hui1; Xia Fang-Bo1; Xiao Zeyu3; hang Xuguang2; Wan Jian-Bo1
2019-05
Source PublicationPHYTOMEDICINE
ISSN0944-7113
Volume58
Abstract

Background
Excess alcohol exposure leads to alcoholic liver disease (ALD). Pueraria lobata (PUE) and Silybum marianum (SIL) are two well-known hepatoprotective herbal remedies with various activities. The possible effect of combination of PUE and SIL on ALD has not been elucidated yet.


Purpose
We aimed to demonstrate that the combination of PUE and SIL prevents against alcoholic liver injury in mice using a model of chronic-plus-single-binge ethanol feeding.


Study design
Male C57BL/6 mice were randomly divided into five groups (n = 8–10), namely the control group (CON), ethanol-induced liver injury group (ETH), 150 mg/kg PUE treated group (PUE), 60 mg/kg SIL treated group (SIL), 210 mg/kg PUE+SIL treatment group (PUE+SIL). Except control group, all animals were fed a modified Lieber-DeCarli ethanol liquid diet for 10 days. While, control group received Lieber-DeCarli control diet containing isocaloric maltose dextrin substituted for ethanol. On day 11, the mice orally received a single dose of 31.5% (v/v) ethanol (5 g/kg BW) or an isocaloric maltose solution.


Results
Ethanol exposure caused liver injury, as demonstrated by remarkably increased plasma parameters, histopathological changes, the increased lipid accumulation, oxidative stress and inflammation in liver. These alterations were ameliorated by the treatments of PUE, SIL and PUE+SIL. While, the PUE+SIL treatment showed the most effective protection, which was associated with reducing alcohol-induced hepatic steatosis via upregulating LKB1/AMPK/ACC signaling, and inhibiting hepatic inflammation via LPS-triggered TLR4-mediated NF-κB signaling pathway. Our results also indicated that the hepatoprotective effects of SIL+PUE might mainly attribute to the protection of SIL and PUE alone in alcohol-induced hepatic steatosis and hepatic inflammation, respectively.


Conclusion
These findings also suggest that the combination of PUE and SIL has a potential to be developed as a functional food for the management of ALD.

KeywordSiL.bum Marianum(L.)Gaerth Pueraria Lobata(Wild.)Ohwi Alcoholic Liver Disease Hepatic Inflammation Intestinal Barrier Dysfunction
DOI10.1016/j.phymed.2019.152824
Indexed BySCI
Language英语
WOS Research AreaPlant Sciences ; Pharmacology & Pharmacy ; Integrative & Complementary Medicine
WOS SubjectPlant Sciences ; Chemistry, Medicinal ; Integrative & Complementary Medicine ; Pharmacology & Pharmacy
WOS IDWOS:000473047100015
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Cited Times [WOS]:3   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Affiliation1.State Key Laboratory of Quality Researchin Chinese Medicine,Institute of Chinese Medical Sciences,University of Macau,Taipa,Macao,China
2.Nutrition and Health Research Centre,By-Health Co.LTD,Guangzhou,China
3.Translational Medicine Collaborative Innovation Center,Department of Pharmacology and Chemical Biology,Institute of Medical Sciences,Shanghai Jiao Tong University School of Medicine,Shanghai,China
First Author AffilicationInstitute of Chinese Medical Sciences
Recommended Citation
GB/T 7714
Feng Ruibing,Chen Jie-Hua,Liu Cong-Hui,et al. A combination of Pueraria lobata and Silybum marianum protects against alcoholic liver disease in mice[J]. PHYTOMEDICINE,2019,58.
APA Feng Ruibing.,Chen Jie-Hua.,Liu Cong-Hui.,Xia Fang-Bo.,Xiao Zeyu.,...&Wan Jian-Bo.(2019).A combination of Pueraria lobata and Silybum marianum protects against alcoholic liver disease in mice.PHYTOMEDICINE,58.
MLA Feng Ruibing,et al."A combination of Pueraria lobata and Silybum marianum protects against alcoholic liver disease in mice".PHYTOMEDICINE 58(2019).
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