UM  > 健康科學學院
Bioevaluation of Ranatuerin-2Pb from the Frog Skin Secretion of Rana pipiens and Its Truncated Analogues
Xiaowei Zhou1,2; Daning Shi2,3; Ruimin Zhong1; Zhuming Ye2; Chengbang Ma2; Mei Zhou2; Xinping Xi2,4; Lei Wang2; Tianbao Chen2; Hang Fai Kwok4
2019-01
Source PublicationBIOMOLECULES
ISSN2218-273X
Volume9Issue:6
Abstract

Antimicrobial peptides (AMPs) are considered as a promising agent to overcome the drug-resistance of bacteria. Large numbers of AMPs have been identified from the skin secretion of Rana pipiens, including brevinins, ranatuerins, temporins and esculentins. In this study, the cDNA precursor of a broad-spectrum antimicrobial peptide, ranatuerin-2Pb, was cloned and identified. Additionally, two truncated analogues, RPa and RPb, were synthesised to investigate the structure-activity relationship of ranatuerin-2Pb. RPa lost antimicrobial activity against Candida albicans, MRSA, Enterococcus faecalis and Pseudomonas aeruginosa, while RPb retained its broad-spectrum antimicrobial activity. Additionally, ranatuerin-2Pb, RPa and RPb demonstrated inhibition and eradication effects against Staphylococcus aureus biofilm. RPb showed a rapid bacterial killing manner via membrane permeabilization without damaging the cell membrane of erythrocytes. Moreover, RPb decreased the mortality of S. aureus infected Galleria mellonella larvae. Collectively, our results suggested that RPb may pave a novel way for natural antimicrobial drug design.

KeywordRanatuerin Antimicrobial Peptides Α-helix Membrane Permeability Waxworm Model
DOI10.3390/biom9060249
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology
WOS SubjectBiochemistry & Molecular Biology
WOS IDWOS:000475301500045
Fulltext Access
Citation statistics
Cited Times [WOS]:2   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionFaculty of Health Sciences
Affiliation1.Department of Nutrition, Henry Fok School of Food Science and Engineering, Shaoguan University, Shaoguan 512005, China
2.Natural Drug Discovery Group, School of Pharmacy, Queen’s University, Belfast BT9 7BL, Northern Ireland, UK
3.School of Government, Peking University, No 114, The Leo KoGuan Building, Beijing 100871, China
4.Institute of Translational Medicine, Faculty of Health Sciences, University of Macau, Avenida de Universidade, Taipa, Macau
Recommended Citation
GB/T 7714
Xiaowei Zhou,Daning Shi,Ruimin Zhong,et al. Bioevaluation of Ranatuerin-2Pb from the Frog Skin Secretion of Rana pipiens and Its Truncated Analogues[J]. BIOMOLECULES,2019,9(6).
APA Xiaowei Zhou.,Daning Shi.,Ruimin Zhong.,Zhuming Ye.,Chengbang Ma.,...&Hang Fai Kwok.(2019).Bioevaluation of Ranatuerin-2Pb from the Frog Skin Secretion of Rana pipiens and Its Truncated Analogues.BIOMOLECULES,9(6).
MLA Xiaowei Zhou,et al."Bioevaluation of Ranatuerin-2Pb from the Frog Skin Secretion of Rana pipiens and Its Truncated Analogues".BIOMOLECULES 9.6(2019).
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Xiaowei Zhou]'s Articles
[Daning Shi]'s Articles
[Ruimin Zhong]'s Articles
Baidu academic
Similar articles in Baidu academic
[Xiaowei Zhou]'s Articles
[Daning Shi]'s Articles
[Ruimin Zhong]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Xiaowei Zhou]'s Articles
[Daning Shi]'s Articles
[Ruimin Zhong]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.