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Insulin Stimulates PI3K/AKT and Cell Adhesion to Promote the Survival of Individualized Human Embryonic Stem Cells
Carlos Godoy‐Parejo1; Chunhao Deng1; Weiwei Liu1,2; Guokai Chen1,2,3
2019-08
Source PublicationSTEM CELLS
ISSN1066-5099
Volume37Issue:8Pages:1030-1041
AbstractInsulin is present in most maintenance media for human embryonic stem cells (hESCs), but little is known about its essential role in the cell survival of individualized cells during passage. In this article, we show that insulin suppresses caspase cleavage and apoptosis after dissociation. Insulin activates insulin‐like growth factor (IGF) receptor and PI3K/AKT cascade to promote cell survival and its function is independent of rho‐associated protein kinase regulation. During niche reformation after passaging, insulin activates integrin that is essential for cell survival. IGF receptor colocalizes with focal adhesion complex and stimulates protein phosphorylation involved in focal adhesion formation. Insulin promotes cell spreading on matrigel‐coated surfaces and suppresses myosin light chain phosphorylation. Further study showed that insulin is also required for the cell survival on E‐cadherin coated surface and in suspension, indicating its essential role in cell–cell adhesion. This work highlights insulin's complex roles in signal transduction and niche re‐establishment in hESCs.
KeywordHesc Cell Survival Cell Adhesion Focal Adhesion Niche Individualization Insulin Integrin Caspase Igf Akt
DOI10.1002/stem.3026
Indexed BySCI
Language英语
WOS Research AreaCell Biology ; Biotechnology & Applied Microbiology ; Oncology ; Hematology
WOS SubjectCell & Tissue Engineering ; Biotechnology & Applied Microbiology ; Oncology ; Cell Biology ; Hematology
WOS IDWOS:000477775400005
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Cited Times [WOS]:3   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionFaculty of Health Sciences
Affiliation1.Centre of Reproduction, Development, and Aging, Faculty of Health Sciences, University of Macau, Macau SAR, People's Republic of China
2.Institute of Translational Medicine, Faculty of Health Sciences, University of Macau, Macau SAR, People's Republic of China
3.Bioimaging and Stem Cell Core Facility, Faculty of Health Sciences, University of Macau, Macau SAR, People's Republic of China
First Author AffilicationFaculty of Health Sciences
Recommended Citation
GB/T 7714
Carlos Godoy‐Parejo,Chunhao Deng,Weiwei Liu,et al. Insulin Stimulates PI3K/AKT and Cell Adhesion to Promote the Survival of Individualized Human Embryonic Stem Cells[J]. STEM CELLS,2019,37(8):1030-1041.
APA Carlos Godoy‐Parejo,Chunhao Deng,Weiwei Liu,&Guokai Chen.(2019).Insulin Stimulates PI3K/AKT and Cell Adhesion to Promote the Survival of Individualized Human Embryonic Stem Cells.STEM CELLS,37(8),1030-1041.
MLA Carlos Godoy‐Parejo,et al."Insulin Stimulates PI3K/AKT and Cell Adhesion to Promote the Survival of Individualized Human Embryonic Stem Cells".STEM CELLS 37.8(2019):1030-1041.
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