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Ratiometric ATP detection on gliding microtubules based on bioorthogonal fluorescence conjugation
Gang Feng; Boyu Zhang; Shichao Wang; Zhaoyang Ding; Gang Li; Xuanjun Zhang
2019-12-12
Source PublicationSensors and Actuators B: Chemical
ISSN0925-4005
Volume301
Abstract

An ingenious microtubule functionalization strategy based on the novel bioorthogonal conjugation is developed to construct fluorescent ratiometric probes for ATP sensing. By single excitation, newly synthesized probe RT-1 exhibited green emission (485 nm) from the bioorthogonal fluorescence conjugation part and orange emission (584 nm) from the ATP sensing part. The fluorescence intensity ratio (I584/I485) displayed good linear response in ATP ranges of 0–2.5 mM (y = 0.9282x + 0.2398, R2 = 0.9705, LOD = 0.0354 mM) and 2.0–10.0 mM (y = 2.7153x-3.6234, R2 = 0.9911, LOD = 0.0121 mM). Moreover, a paclitaxel derivate probe RT-2 was developed to functionalize in vitro polymerized microtubule for in situ ATP detection. This simple and convenient strategy is anticipated to stimulate more microtubule related applications in both in vitro and intracellular studies.

KeywordBioorthogonal Fluorescence Inverse Electron Demand Diels–alder Reaction Atp Microtubule Ratiometric Sensing
DOI10.1016/j.snb.2019.127090
Indexed BySCIE
Language英语
WOS Research AreaChemistry ; Electrochemistry ; Instruments & Instrumentation
WOS SubjectChemistry, Analytical ; Electrochemistry ; Instruments & Instrumentation
WOS IDWOS:000489529300074
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Cited Times [WOS]:1   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionFaculty of Health Sciences
Corresponding AuthorXuanjun Zhang
AffiliationCancer Centre and Center for Precision Medicine Research and Training, Faculty of Health Sciences, University of Macau, Macau SAR, 999078, China
First Author AffilicationFaculty of Health Sciences
Corresponding Author AffilicationFaculty of Health Sciences
Recommended Citation
GB/T 7714
Gang Feng,Boyu Zhang,Shichao Wang,et al. Ratiometric ATP detection on gliding microtubules based on bioorthogonal fluorescence conjugation[J]. Sensors and Actuators B: Chemical,2019,301.
APA Gang Feng,Boyu Zhang,Shichao Wang,Zhaoyang Ding,Gang Li,&Xuanjun Zhang.(2019).Ratiometric ATP detection on gliding microtubules based on bioorthogonal fluorescence conjugation.Sensors and Actuators B: Chemical,301.
MLA Gang Feng,et al."Ratiometric ATP detection on gliding microtubules based on bioorthogonal fluorescence conjugation".Sensors and Actuators B: Chemical 301(2019).
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