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Applications of Protein Fragment Complementation Assays for Analyzing Biomolecular Interactions and Biochemical Networks in Living Cells
Peipei Li1; Li Wang1,2; Li-jun Di1
2019-06-24
Source PublicationJOURNAL OF PROTEOME RESEARCH
ISSN1535-3893
Volume18Issue:8Pages:2987-2998
AbstractProtein-protein interactions (PPIs) are indispensable for the dynamic assembly of multiprotein complexes that are central players of nearly all of the intracellular biological processes, such as signaling pathways, metabolic pathways, formation of intracellular organelles, establishment of cytoplasmic skeletons, etc. Numerous approaches have been invented to study PPIs both in vivo and in vitro, including the protein-fragment complementation assay (PCA), which is a widely applied technology to study PPIs and biomolecular interactions. PCA is a technology based on the expression of the bait and prey proteins in fusion with two complementary reporter protein fragments, respectively, that will reassemble when in close proximity. The reporter protein can be the enzymes or fluorescent proteins. Recovery of the enzymatic activity or fluorescent signal can be the indicator of PPI between the bait and prey proteins. Significant effort has been invested in developing many derivatives of PCA, along with various applications, in order to address specific questions. Therefore, a prompt review of these applications is important. In this review, we will categorize these applications according to the scenarios that the PCAs were applied and expect to provide a reference guideline for the future selection of PCA methods in solving a specific problem.
KeywordProtein-fragment Complementation Assays Bifc Biomolecular Interactions Biochemical Network Split Enzymes
DOI10.1021/acs.jproteome.9b00154
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology
WOS SubjectBiochemical Research Methods
WOS IDWOS:000480370800001
PublisherAMER CHEMICAL SOC, 1155 16TH ST, NW, WASHINGTON, DC 20036 USA
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Cited Times [WOS]:2   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionFaculty of Health Sciences
Corresponding AuthorLi-jun Di
Affiliation1.Cancer Center, Faculty of Health Sciences, University of Macau, Macau, SAR of China
2.Metabolomics Core, Faculty of Health Sciences, University of Macau, Macau, SAR of China
First Author AffilicationFaculty of Health Sciences
Corresponding Author AffilicationFaculty of Health Sciences
Recommended Citation
GB/T 7714
Peipei Li,Li Wang,Li-jun Di. Applications of Protein Fragment Complementation Assays for Analyzing Biomolecular Interactions and Biochemical Networks in Living Cells[J]. JOURNAL OF PROTEOME RESEARCH,2019,18(8):2987-2998.
APA Peipei Li,Li Wang,&Li-jun Di.(2019).Applications of Protein Fragment Complementation Assays for Analyzing Biomolecular Interactions and Biochemical Networks in Living Cells.JOURNAL OF PROTEOME RESEARCH,18(8),2987-2998.
MLA Peipei Li,et al."Applications of Protein Fragment Complementation Assays for Analyzing Biomolecular Interactions and Biochemical Networks in Living Cells".JOURNAL OF PROTEOME RESEARCH 18.8(2019):2987-2998.
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