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Requirements for RNA polymerase II preinitiation complex formation in vivo
Natalia Petrenko1; Yi Jin1; Liguo Dong2; Koon Ho Wong3; Kevin Struhl1
2019-01-25
Source PublicationELIFE
ISSN2050-084X
Volume8
Abstract

Transcription by RNA polymerase II requires assembly of a preinitiation complex (PIC) composed of general transcription factors (GTFs) bound at the promoter. In vitro, some GTFs are essential for transcription, whereas others are not required under certain conditions. PICs are stable in the absence of nucleotide triphosphates, and subsets of GTFs can form partial PICs. By depleting individual GTFs in yeast cells, we show that all GTFs are essential for TBP binding and transcription, suggesting that partial PICs do not exist at appreciable levels in vivo. Depletion of FACT, a histone chaperone that travels with elongating Pol II, strongly reduces PIC formation and transcription. In contrast, TBP-associated factors (TAFs) contribute to transcription of most genes, but TAF-independent transcription occurs at substantial levels, preferentially at promoters containing TATA elements. PICs are absent in cells deprived of uracil, and presumably UTP, suggesting that transcriptionally inactive PICs are removed from promoters in vivo.

DOI10.7554/eLife.43654
Indexed BySCIE
Language英语
WOS Research AreaLife Sciences & Biomedicine - Other Topics
WOS SubjectBiology
WOS IDWOS:000458102700001
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Cited Times [WOS]:2   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionFaculty of Health Sciences
Corresponding AuthorKoon Ho Wong; Kevin Struhl
Affiliation1.Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, United States
2.Faculty of Health Sciences, University of Macau, Macau, China
3.Institute of Translational Medicine, University of Macau, Macau, China
Corresponding Author AffilicationUniversity of Macau
Recommended Citation
GB/T 7714
Natalia Petrenko,Yi Jin,Liguo Dong,et al. Requirements for RNA polymerase II preinitiation complex formation in vivo[J]. ELIFE,2019,8.
APA Natalia Petrenko,Yi Jin,Liguo Dong,Koon Ho Wong,&Kevin Struhl.(2019).Requirements for RNA polymerase II preinitiation complex formation in vivo.ELIFE,8.
MLA Natalia Petrenko,et al."Requirements for RNA polymerase II preinitiation complex formation in vivo".ELIFE 8(2019).
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