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Long-term in vivo monitoring of injury induced brain regeneration of the adult zebrafish by using spectral domain optial coherence tomography
Zhen Yuan; Jian Zhang
2018
Conference NameConference on Dynamics and Fluctuations in Biomedical Photonics XV
Source PublicationDYNAMICS AND FLUCTUATIONS IN BIOMEDICAL PHOTONICS XV
Volume10493
Conference DateJAN 28-29, 2018
Conference PlaceSan Francisco, CA
Abstract

The adult zebrafish has pronounced regenerative capacity of the brain, which makes it an ideal model organism of vertebrate biology for the investigation of recovery of central nervous system injuries. The aim of this study was to employ spectral-domain optical coherence tomography (SD-OCT) system for long-term in vivo monitoring of tissue regeneration using an adult zebrafish model of brain injury. Based on a 1325 nm light source and two high-speed galvo mirrors, the SD-OCT system can offer a large field of view of the three-dimensional (3D) brain structures with high imaging resolution (12 mu m axial and 13 mu m lateral) at video rate. In vivo experiments based on this system were conducted to monitor the regeneration process of zebrafish brain after injury during a period of 43 days. To monitor and detect the process of tissue regeneration, we performed 3D in vivo imaging in a zebrafish model of adult brain injury during a period of 43 days. The coronal and sagittal views of the injured zebrafish brain at each time point (0 days, 10 days, 20 days and 43 days post-lesion) were presented to show the changes of the brain lesion in detail. In addition, the 3D SD-OCT images for an injured zebrafish brain were also reconstructed at days 0 and days 43 post-lesion. We found that SD-OCT is able to effectively and non-invasively monitor the regeneration of the adult zebrafish brain after injury in real time with high 3D spatial resolution and good penetration depth. Our findings also suggested that the adult zebrafish has the extraordinary capability of brain regeneration and is able to repair itself after brain injury.

KeywordBrain Injury Regeneration Zebrafish Optical Coherence Tomography
DOI10.1117/12.2286798
Indexed ByCPCI
Language英语
WOS Research AreaOptics ; Radiology, Nuclear Medicine & Medical Imaging
WOS SubjectOptics ; Radiology, Nuclear Medicine & Medical Imaging
WOS IDWOS:000454728100007
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Document TypeConference paper
CollectionFaculty of Health Sciences
INSTITUTE OF COLLABORATIVE INNOVATION
AffiliationUniversity of Macau, Bioimaging Core, Faculty of Health Sciences, Macau SAR, China
First Author AffilicationFaculty of Health Sciences
Recommended Citation
GB/T 7714
Zhen Yuan,Jian Zhang. Long-term in vivo monitoring of injury induced brain regeneration of the adult zebrafish by using spectral domain optial coherence tomography[C],2018.
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