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Revisiting Fragmentation Reactions of Protonated alpha-Amino Acids by High-Resolution Electrospray lonization Tandem Mass Spectrometry with Collision-Induced Dissociation
Pengwei Zhang1; Wan Chan2; Irene L. Ang1; Rui Wei1; Melody M. T. Lam3; Kate M. K. Lei1; Terence C. W. Poon1
2019-04-23
Source PublicationSCIENTIFIC REPORTS
ISSN2045-2322
Volume9
Abstract

Fragmentation reactions of protonated alpha-amino acids (AAs) were studied previously using tandem mass spectrometry (MS/MS) of unit mass resolution. Isobaric fragmentation products and minor fragmentation products could have been overlooked or misannotated. In the present study, we examined the fragmentation patterns of 19 AAs using high-resolution electrospray ionization MS/MS (HR-ESI-MS/MS) with collision-induced dissociation (CID). Isobaric fragmentation products from protonated Met and Trp were resolved and identified for the first time. Previously unreported fragmentation products from protonated Met, Cys, Gln, Arg, and Lys were observed. Additionally, the chemical identity of a fragmentation product from protonated Trp that was incorrectly annotated in previous investigations was corrected. All previously unreported fragmentation products and reactions were verified by pseudo MS3 experiments and/or MS/MS analyses of deuterated AAs. Clearer pictures of the fragmentation reactions for Met, Cys, Trp, Gin, Arg and Lys were obtained in the present study.

DOI10.1038/s41598-019-42777-8
Indexed BySCIE
Language英语
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS IDWOS:000465218400004
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Cited Times [WOS]:4   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionFaculty of Health Sciences
Corresponding AuthorTerence C. W. Poon
Affiliation1.Pilot Laboratory, Institute of Translational Medicine, Faculty of Health Sciences, University of Macau, Macau, China
2.Department of Chemistry, Hong Kong University of Science and Technology, Hong Kong, China
3.Proteomics Core, Faculty of Health Sciences, University of Macau, Macau, China
First Author AffilicationUniversity of Macau
Corresponding Author AffilicationUniversity of Macau
Recommended Citation
GB/T 7714
Pengwei Zhang,Wan Chan,Irene L. Ang,et al. Revisiting Fragmentation Reactions of Protonated alpha-Amino Acids by High-Resolution Electrospray lonization Tandem Mass Spectrometry with Collision-Induced Dissociation[J]. SCIENTIFIC REPORTS,2019,9.
APA Pengwei Zhang.,Wan Chan.,Irene L. Ang.,Rui Wei.,Melody M. T. Lam.,...&Terence C. W. Poon.(2019).Revisiting Fragmentation Reactions of Protonated alpha-Amino Acids by High-Resolution Electrospray lonization Tandem Mass Spectrometry with Collision-Induced Dissociation.SCIENTIFIC REPORTS,9.
MLA Pengwei Zhang,et al."Revisiting Fragmentation Reactions of Protonated alpha-Amino Acids by High-Resolution Electrospray lonization Tandem Mass Spectrometry with Collision-Induced Dissociation".SCIENTIFIC REPORTS 9(2019).
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