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Gas-Phase Fragmentation Reactions of Protonated Cystine using High-Resolution Tandem Mass Spectrometry
Pengwei Zhang1; Wan Chan2; Irene L. Ang1; Rui Wei1; Melody M. T. Lam3; Kate M. K. Lei1; Terence C. W. Poon1
2019-02-02
Source PublicationMOLECULES
ISSN1420-3049
Volume24Issue:4
Abstract

Cystine is an important biomolecule in living systems. Although collision-induced dissociation (CID)-based tandem mass spectrometry (MS/MS) is commonly applied for identification and quantification of cystine in both biomedical and nutritional studies, gas-phase fragmentation reactions of cystine in CID has remained unclear. This may lead to improper assay design, which may in turn result in inaccurate test results. In the present study, gas-phase fragmentation reactions of protonated cystine in CID were characterized using high-resolution MS/MS and pseudo MS3. Fragmentations started from cleavages of disulfide bond (S–S) and carbon–sulfur bond (C–S). When cleaving at the S–S, protonated cysteine was generated as one of the predominant fragmentation products. Minor fragmentations started from the loss of H2O + CO and the loss of NH3. Our results reveal that the m/z 74 fragment ion, which is commonly used as a product ion of the transition (precursor/product ion pair) in selected reaction monitoring (SRM) assay for quantifying cystine, comprises two isobaric fragments originating from different parts of cystine. This indicates the need for careful selection of a stable isotope-labeled cystine molecule as an internal standard for SRM assays. Here, we provide a clear picture of the fragmentation reactions of protonated cystine in CID. It can serve as a useful guidance for designing MS/MS-based assays for cystine testing.

KeywordCystine Gas-phase Fragmentation Reaction High-resolution Ms Pseudo Ms3 Isobaric Fragment Ms Cysteine
DOI10.3390/molecules24040747
Indexed BySCIE
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS SubjectBiochemistry & Molecular Biology ; Chemistry, Multidisciplinary
WOS IDWOS:000460805900093
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Document TypeJournal article
CollectionFaculty of Health Sciences
Corresponding AuthorTerence C. W. Poon
Affiliation1.Pilot Laboratory, Institute of Translational Medicine, Faculty of Health Sciences, University of Macau, Macau, China
2.Department of Chemistry, Hong Kong University of Science and Technology, Hong Kong, China
3.Proteomics Core, Faculty of Health Sciences, University of Macau, Macau, China
First Author AffilicationFaculty of Health Sciences
Corresponding Author AffilicationFaculty of Health Sciences
Recommended Citation
GB/T 7714
Pengwei Zhang,Wan Chan,Irene L. Ang,et al. Gas-Phase Fragmentation Reactions of Protonated Cystine using High-Resolution Tandem Mass Spectrometry[J]. MOLECULES,2019,24(4).
APA Pengwei Zhang.,Wan Chan.,Irene L. Ang.,Rui Wei.,Melody M. T. Lam.,...&Terence C. W. Poon.(2019).Gas-Phase Fragmentation Reactions of Protonated Cystine using High-Resolution Tandem Mass Spectrometry.MOLECULES,24(4).
MLA Pengwei Zhang,et al."Gas-Phase Fragmentation Reactions of Protonated Cystine using High-Resolution Tandem Mass Spectrometry".MOLECULES 24.4(2019).
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