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Oridonin loaded solid lipid nanoparticles enhanced antitumor activity in MCF-7 cells
Lu Wang1; Shengpeng Wang1; Ruie Chen1; Yanping Wang2; Hui Li2; Yitao Wang1; Meiwan Chen1
2014
Source PublicationJournal of Nanomaterials
ISSN1687-4110
Volume2014
Abstract

Oridonin (ORI), a famous diterpenoid from Chinese herbal medicine, has drawn rising attention for its remarkable apoptosis and autophagy-inducing activity in human cancer therapy, while clinical application of ORI is limited by its strong hydrophobicity and rapid plasma clearance. The purpose of this study was to evaluate whether the antitumor activity of ORI could be enhanced by loading into solid lipid nanoparticles (SLNs). ORI-loaded SLNs were prepared by hot high pressure homogenization with narrow size distribution and good entrapment efficacy. MTT assay indicated that ORI-loaded SLNs enhanced the inhibition of proliferation against several human cancer cell lines including breast cancer MCF-7 cells, hepatocellular carcinoma HepG 2 cells, and lung carcinoma A549 cells compared with free ORI, while no significant enhancement of toxicity to human mammary epithelial MCF-10A cells was shown. Meanwhile, flow cytometric analysis demonstrated that ORI-SLNs induced more significant cell cycle arrest at S and decreased cell cycle arrest at G1/G0 phase in MCF-7 cells than bulk ORI solution. Hoechst 33342 staining and Annexin V/PI assay indicated that apoptotic rates of cells treated with ORI-loaded SLNs were higher compared with free ORI. In summary, our data indicated that SLNs may be a potential carrier for enhancing the antitumor effect of hydrophobic drug ORI. © 2014 Lu Wang et al.

DOI10.1155/2014/903646
URLView the original
Indexed BySCIE
Language英语
WOS Research AreaScience & Technology - Other Topics ; Materials Science
WOS SubjectNanoscience & Nanotechnology ; Materials Science, Multidisciplinary
WOS IDWOS:000334245300001
The Source to ArticleScopus
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Cited Times [WOS]:10   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Affiliation1.State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macau 999078, Macau
2.Institute of Chinese Materia Medica, Chinese Academy of Chinese Medical Sciences, Beijing 100700, China
First Author AffilicationInstitute of Chinese Medical Sciences
Recommended Citation
GB/T 7714
Lu Wang,Shengpeng Wang,Ruie Chen,et al. Oridonin loaded solid lipid nanoparticles enhanced antitumor activity in MCF-7 cells[J]. Journal of Nanomaterials,2014,2014.
APA Lu Wang.,Shengpeng Wang.,Ruie Chen.,Yanping Wang.,Hui Li.,...&Meiwan Chen.(2014).Oridonin loaded solid lipid nanoparticles enhanced antitumor activity in MCF-7 cells.Journal of Nanomaterials,2014.
MLA Lu Wang,et al."Oridonin loaded solid lipid nanoparticles enhanced antitumor activity in MCF-7 cells".Journal of Nanomaterials 2014(2014).
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