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Anti-neuroinflammatory effects of SLOH in Aβ-induced BV-2 microglial cells and 3xTg-AD mice involve the inhibition of GSK-3β
Wu X.; Kosaraju J.; Tam K.Y.
2018-11-20
Source PublicationNeuroscience Letters
ISSN18727972 03043940
Volume687Pages:207-215
AbstractNeuroinflammation has been observed in post-mortem Alzheimer's disease (AD) brains which could be due to Aβ interacting with microglia and astrocytes. SLOH, a carbazole-based fluorophore, was shown to bind to Aβ peptides. Herein, we investigated the anti-neuroinflammatory effects of SLOH using a BV-2 microglial cell model and a triple transgenic AD (3xTg-AD) mouse model. BV-2 cells were incubated with Aβ in the presence of SLOH for 24 h. The levels of pro-inflammatory and anti-inflammatory cytokines were determined. Moreover, 3xTg-AD mice were administrated with SLOH (2 mg kg) for one month. The mice were then sacrificed and the brains were used to assess the levels of pro-inflammatory, anti-inflammatory cytokines and the activation of ionized calcium-binding adapter molecule 1 (Iba1). BV-2 cell studies suggested that SLOH reduced the production and mRNA levels of pro-inflammatory cytokines TNF-α, IL-1β, COX-2, iNOS, and increased IL-10. Animal study confirmed that SLOH reduced the production of pro-inflammatory cytokines and increased the level of anti-inflammatory cytokine. Moreover, SLOH inhibited the activity of GSK-3β. In 3xTg-AD mouse model, SLOH treatment significantly decreased the number of Iba1-positive cells in mouse brains. Our results demonstrated that SLOH significantly attenuated the neuroinflammation through down-regulating the activity of GSK-3β.
Keyword3xTg-AD mice BV-2 cells GSK-3β Neuroinflammation SLOH
DOI10.1016/j.neulet.2018.09.056
URLView the original
Language英語
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Cited Times [WOS]:2   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
AffiliationUniversidade de Macau
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Wu X.,Kosaraju J.,Tam K.Y.. Anti-neuroinflammatory effects of SLOH in Aβ-induced BV-2 microglial cells and 3xTg-AD mice involve the inhibition of GSK-3β[J]. Neuroscience Letters,2018,687:207-215.
APA Wu X.,Kosaraju J.,&Tam K.Y..(2018).Anti-neuroinflammatory effects of SLOH in Aβ-induced BV-2 microglial cells and 3xTg-AD mice involve the inhibition of GSK-3β.Neuroscience Letters,687,207-215.
MLA Wu X.,et al."Anti-neuroinflammatory effects of SLOH in Aβ-induced BV-2 microglial cells and 3xTg-AD mice involve the inhibition of GSK-3β".Neuroscience Letters 687(2018):207-215.
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