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Multiphoton imaging to distinguish grana and starch inside an intact leaf
Chen M.-Y.; Zhuo G.-Y.; Chen P.-F.; Wu P.-C.; Liu T.-M.; Chu S.-W.
2013-06-12
Conference NameConference on Multiphoton Microscopy in the Biomedical Sciences XIII
Source PublicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume8588
Conference DateFEB 03-05, 2013
Conference PlaceSan Francisco, CA
Abstract

We have demonstrated a straightforward and noninvasive method to identify the distribution of grana and starch within an intact leaf. Grana and starch are the major functional structures for photosynthesis and energy storage of plant, respectively. Both exhibit highly ordered molecular structures and appear as micrometer-sized granules inside chloroplasts. In order to distinguish grana and starch, we used multiphoton microscopy, with simultaneous acquisition of two photon fluorescence (2PF) and second harmonic generation (SHG) signals. Consequently, SHG is found on both grana and starch while 2PF from chlorophyll indicates the identity of grana. © 2013 SPIE.

KeywordGrana Second Harmonic Generation Starch Two Photon Fluorescence
DOI10.1117/12.2003075
URLView the original
Indexed BySCI
Language英语
WOS Research AreaEngineering ; Microscopy ; Optics
WOS SubjectEngineering, Biomedical ; Microscopy ; Optics
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Cited Times [WOS]:0   [WOS Record]     [Related Records in WOS]
Document TypeConference paper
CollectionFaculty of Health Sciences
AffiliationNational Taiwan University
Recommended Citation
GB/T 7714
Chen M.-Y.,Zhuo G.-Y.,Chen P.-F.,et al. Multiphoton imaging to distinguish grana and starch inside an intact leaf[C],2013.
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