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Online comprehensive two-dimensional hydrophilic interaction chromatography×reversed-phase liquid chromatography coupled with hybrid linear ion trap Orbitrap mass spectrometry for the analysis of phenolic acids in Salvia miltiorrhiza
Cao J.-L.; Wang S.-S.; Hu H.; He C.-W.; Wan J.-B.; Su H.-X.; Wang Y.-T.; Li P.
2018-02-09
Source PublicationJournal of chromatography. A
ISSN18733778
Volume1536Pages:216-227
AbstractIn the present study, a system was developed for the analysis of phenolic acids in Salvia miltiorrhiza using online comprehensive two-dimensional hydrophilic interaction chromatography and reversed-phase liquid chromatography coupled to a DAD detector and hybrid linear ion trap-Orbitrap mass spectrometry (HILIC×RP-DAD-ESI/HRMS/MSn). The system was configured based on the combination of an XBridge Amide column (150mm×4.6mm, 3.5μm) and Accucore PFP column (50mm×4.6mm, 2.6μm) for the first and second dimensions, respectively. An additional LC pump was used to dilute the eluent from the first dimension to decrease its elution strength in the second dimension. A back-flush trap column was selected as an interface to make up for the loss of efficiency and resolution due to the online dilution. Under the optimized conditions, a total of 196 peaks of polar compounds were successfully separated and detected in Salvia miltiorrhiza using the developed online HILIC×RP system, which exhibited high orthogonality (73%). The online combination of HILIC and RP provides powerful separation capability for the analysis of polar compounds in samples with complex matrices.
KeywordDanshen Interfaces LTQ-Orbitrap mass spectrometry Online HILIC×RPLC Phenolic acids
DOI10.1016/j.chroma.2017.09.041
URLView the original
Language英語
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Cited Times [WOS]:14   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
AffiliationUniversidade de Macau
Recommended Citation
GB/T 7714
Cao J.-L.,Wang S.-S.,Hu H.,et al. Online comprehensive two-dimensional hydrophilic interaction chromatography×reversed-phase liquid chromatography coupled with hybrid linear ion trap Orbitrap mass spectrometry for the analysis of phenolic acids in Salvia miltiorrhiza[J]. Journal of chromatography. A,2018,1536:216-227.
APA Cao J.-L..,Wang S.-S..,Hu H..,He C.-W..,Wan J.-B..,...&Li P..(2018).Online comprehensive two-dimensional hydrophilic interaction chromatography×reversed-phase liquid chromatography coupled with hybrid linear ion trap Orbitrap mass spectrometry for the analysis of phenolic acids in Salvia miltiorrhiza.Journal of chromatography. A,1536,216-227.
MLA Cao J.-L.,et al."Online comprehensive two-dimensional hydrophilic interaction chromatography×reversed-phase liquid chromatography coupled with hybrid linear ion trap Orbitrap mass spectrometry for the analysis of phenolic acids in Salvia miltiorrhiza".Journal of chromatography. A 1536(2018):216-227.
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