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Identification and quantification of the anti-inflammatory constituents in Pian-Tze-Huang by liquid chromatography combined with quadrupole time-of-flight and triple quadrupole mass spectrometry
Huang M.1; Xu W.1; Zhang Y.2; Liu J.1; Zhang X.1; Lin J.1; Peng J.1; Lu J.3
2016-08-01
Source PublicationJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
ISSN1873376X 15700232
Volume1027Pages:27-39
Abstract

Both qualitative and quantitative analyses combined with activity evaluation were used for the integrated quality control study of Pien-Tze-Huang (PZH), a precious lozenge used for 460 years in China. In qualitative analysis, a high performance LC-quadrupole time-of-flight MS method was developed for the identification of chemical profiling in PZH. 27 compounds were unambiguously identified by comparing with reference substances, and 12 compounds were tentatively deduced based on their MS data and comparing with literatures. Then the in vitro anti-inflammatory activity of 27 compounds were evaluated, and 9 saponins, 11 bile acids, taurine and muscone exhibited significant inhibitory activities on TNF-α production with IC values ranging from 12.34 to 147.24 μM. In quantitative analysis, 21 active compounds, selected as quality control markers, were simultaneously detected in 15 batches of PZH samples by ultra-performance LC-triple quadrupole MS. All the analytes were detected within 15 min. Satisfactory linearity was achieved with wide linear range and fine determination coefficient (r > 0.9990). The respective relative standard deviations of intra- and inter-day precisions, repeatability, stability, and recovery were less than 4.32%, 4.94%, 4.78%, 4.87%, and 4.91%. This study established a high sensitive and efficient method for the integrated quality control of PZH, including qualitative and quantitative analyses combined with pharmacological activity evaluation.

KeywordAnti-inflammatory Activity Identification And Quantification Mass Spectrometry Pian-tze-huang Quality Control
DOI10.1016/j.jchromb.2016.05.021
URLView the original
Indexed BySCI
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS IDWOS:000379636900004
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Cited Times [WOS]:6   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Affiliation1.Fujian University of Traditional Chinese Medicine
2.State Oceanic Administration China
3.Universidade de Macau
Recommended Citation
GB/T 7714
Huang M.,Xu W.,Zhang Y.,et al. Identification and quantification of the anti-inflammatory constituents in Pian-Tze-Huang by liquid chromatography combined with quadrupole time-of-flight and triple quadrupole mass spectrometry[J]. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences,2016,1027:27-39.
APA Huang M..,Xu W..,Zhang Y..,Liu J..,Zhang X..,...&Lu J..(2016).Identification and quantification of the anti-inflammatory constituents in Pian-Tze-Huang by liquid chromatography combined with quadrupole time-of-flight and triple quadrupole mass spectrometry.Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences,1027,27-39.
MLA Huang M.,et al."Identification and quantification of the anti-inflammatory constituents in Pian-Tze-Huang by liquid chromatography combined with quadrupole time-of-flight and triple quadrupole mass spectrometry".Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences 1027(2016):27-39.
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