UM
Development and validation of a rapid RP-HPLC-DAD analysis method for the quantification of pilocarpine in Pilocarpus microphyllus (Rutaceae)
Caldas Pereira R.1; Nonato C.D.F.A.1; Camilo C.J.1; Melo Coutinho H.D.1; Rodrigues F.F.G.1; Xiao J.2; da Costa J.G.M.1
2018-09-01
Source PublicationFood and Chemical Toxicology
ISSN18736351 02786915
Volume119Pages:106-111
Abstract

A method using high performance liquid chromatography with diode array detector (HPLC-DAD) for identification and quantification of pilocarpine in the extract of Pilocarpus microphyllus, popularly known as jaborandi. The analysis was conducted using RP-18 column (250 mm × 4.6 mm x 5 μm id) and a buffer solution composed of acidified water, phosphoric acid and triethylamine and methanol as a mobile phase in a flow rate of 1.0 mL/min and detection at 215 nm at 25 °C. Excellent linearity with r equal to 0.9999 was obtained. The recovery percentage was very satisfactory with values within the specifications. It is correct to affirm that the method has optimal intracurrent and intercurrent precision values with relative standard deviations of 0.1852% and 0.1932%, respectively. The robustness of the method, assessed through the Youden test, showed no significant influence of any of the evaluated parameters. In general, the method proved to be suitable for the intended purpose.

KeywordAlkaloid Hplc Pilocarpine Pilocarpus Microphyllus Quantification Validation
DOI10.1016/j.fct.2018.05.023
URLView the original
Indexed BySCIE ; CPCI
WOS Research AreaFood Science & Technology ; Toxicology
WOS SubjectFood Science & Technology ; Toxicology
WOS IDWOS:000443664200014
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Cited Times [WOS]:3   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
Affiliation1.Universidade Regional do Cariri
2.Universidade de Macau
Recommended Citation
GB/T 7714
Caldas Pereira R.,Nonato C.D.F.A.,Camilo C.J.,et al. Development and validation of a rapid RP-HPLC-DAD analysis method for the quantification of pilocarpine in Pilocarpus microphyllus (Rutaceae)[J]. Food and Chemical Toxicology,2018,119:106-111.
APA Caldas Pereira R..,Nonato C.D.F.A..,Camilo C.J..,Melo Coutinho H.D..,Rodrigues F.F.G..,...&da Costa J.G.M..(2018).Development and validation of a rapid RP-HPLC-DAD analysis method for the quantification of pilocarpine in Pilocarpus microphyllus (Rutaceae).Food and Chemical Toxicology,119,106-111.
MLA Caldas Pereira R.,et al."Development and validation of a rapid RP-HPLC-DAD analysis method for the quantification of pilocarpine in Pilocarpus microphyllus (Rutaceae)".Food and Chemical Toxicology 119(2018):106-111.
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