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Ratiometric fluorescent detection of pesticide based on split aptamer and magnetic separation
Wang, Ying1; Zhu, Fuyan1; Yin, Li1; Qu, Guangxu1; Ma, Dik Lung2; Leung, Chung Hang3; Lu, Lihua1
Source PublicationSensors and Actuators B: Chemical

Pesticide residues have seriously threatened human health and affected the sustainable development of the environment. It is pivotal to establish an efficient detection platform for pesticide assays. A novel ratiometric fluorescent aptasensor for sensitive determination of pesticide has been successfully established. Carbon dots (CDs) are used as the reference emitter, while the T-rich single-stranded DNA templated copper nanoparticles (CuNPs) provide the increasing signal. More precisely, CDs/magnetic beads (MBs) combined with split aptamer are used in this detection platform to present a label-free and enzyme-free isocarbophos (ICP) detection with high sensitivity and specificity. In the presence of ICP, a sandwich-typed complex is formed which attaches a T-rich DNA. After magnetic separation and addition of Cu/ascorbic acid (AA), the CuNPs are generated and the strong red luminescent appears. Without ICP, just blue emission from CDs exits. The developed ratiometric aptasensor offers an excellent ICP detection in the linear range of 3–200 nM with a detection limit of 0.81 nM. It provides new ways for the assay of pesticides in agricultural products or environmental samples.

KeywordAptasensor Carbon Dot Cunps Pesticide Ratiometric Split Aptamer
URLView the original
Indexed BySCIE
WOS Research AreaChemistry ; Electrochemistry ; Instruments & Instrumentation
WOS SubjectChemistry, Analytical ; Electrochemistry ; Instruments & Instrumentation
WOS IDWOS:000829391900001
Scopus ID2-s2.0-85130515596
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Cited Times [WOS]:1   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Corresponding AuthorMa, Dik Lung; Leung, Chung Hang; Lu, Lihua
Affiliation1.College of Chemistry and Pharmaceutical Sciences, Qingdao Agricultural University, Qingdao, 266109, China
2.Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, 999077, Hong Kong
3.State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, 999078, Macao
Corresponding Author AffilicationInstitute of Chinese Medical Sciences
Recommended Citation
GB/T 7714
Wang, Ying,Zhu, Fuyan,Yin, Li,et al. Ratiometric fluorescent detection of pesticide based on split aptamer and magnetic separation[J]. Sensors and Actuators B: Chemical,2022,367.
APA Wang, Ying,Zhu, Fuyan,Yin, Li,Qu, Guangxu,Ma, Dik Lung,Leung, Chung Hang,&Lu, Lihua.(2022).Ratiometric fluorescent detection of pesticide based on split aptamer and magnetic separation.Sensors and Actuators B: Chemical,367.
MLA Wang, Ying,et al."Ratiometric fluorescent detection of pesticide based on split aptamer and magnetic separation".Sensors and Actuators B: Chemical 367(2022).
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