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Identification of AHSA1 as a Potential Therapeutic Target for Breast Cancer: Bioinformatics Analysis and in vitro Studies
Shi, Wei1; Qi, Lu1; You, Xiong Bin2; Chen, Yu Chi1; Xu, Yu Lian1; Yu, Wei Bang1; Huang, Mu Yang1; Zhao, Hong2; Lu, Jin Jian1,3,4
2022-02-14
Source PublicationCurrent Cancer Drug Targets
ISSN1568-0096
Volume22Issue:2Pages:142-152
Abstract

Background: Shenling Baizhu Powder (SBP), a famous Traditional Chinese Medicine (TCM) formulation, has been widely used in the adjuvant treatment of cancers, including breast cancer. This study aims to identify potential new targets for breast cancer treatment based on the network pharmacology of SBP. Methods: By analyzing the relationship between herbs and target proteins, potential targets of multiple herbs in SBP were identified by network pharmacology analysis. Besides, by comparing the data of breast cancer tissue with normal tissue, upregulated genes in two breast cancer expression profiles were found. Thereafter, the expression level and prognosis of activator of heat shock protein 90 (HSP90) ATPase activity 1 (AHSA1) were further analyzed in breast cancer by bioinformatics analysis, and the network module of AHSA1 binding protein was constructed. Furthermore, the effect of knocking down AHSA1 on the proliferation, migration, and invasion of breast cancer cells was verified by MTT, clone formation assay, and transwell assay. Results: Vascular endothelial growth factor A (VEGFA), intercellular adhesion molecule 1 (ICAM1), chemokine (C-X-C motif) ligand 8 (CXCL8), AHSA1, and serpin family E member 1 (SERPINE1) were associated with multiple herbs in SBP. AHSA1 was remarkably upregulated in breast cancer tissues and positively correlated with poor overall survival and disease metastasis-free survival. Furthermore, knockdown of AHSA1 significantly inhibited the migration and invasion in MCF-7 and MDA-MB-231 breast cancer cells but had no obvious effect on proliferation. In addition, among the proteins that bind to AHSAl, the network composed of proteasome, chaperonin, and heat shock proteins is closely connected, and these proteins are associated with poor prognosis in a variety of cancers. Conclusion: AHSA1 is positively correlated with breast cancer progression and might act as a novel therapeutic target for breast cancer.

KeywordAhsa1 Breast Cancer Invasion Migration Network Pharmacology Shenling Baizhu Powder
DOI10.2174/1568009622666220114151058
URLView the original
Language英語English
Scopus ID2-s2.0-85130861906
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Document TypeJournal article
CollectionFaculty of Social Sciences
Institute of Chinese Medical Sciences
Corresponding AuthorLu, Jin Jian
Affiliation1.State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao
2.Department of Breast Surgery, The First Affiliated Hospital of Zhejiang, Chinese Medical University, Hangzhou, 310006, China
3.Department of Pharmaceutical Sciences, Faculty of Health Sciences, University of Macau, Macao
4.MoE Frontiers Science Center for Precision Oncology, University of Macau, Macao
First Author AffilicationInstitute of Chinese Medical Sciences
Corresponding Author AffilicationInstitute of Chinese Medical Sciences;  Faculty of Health Sciences;  University of Macau
Recommended Citation
GB/T 7714
Shi, Wei,Qi, Lu,You, Xiong Bin,et al. Identification of AHSA1 as a Potential Therapeutic Target for Breast Cancer: Bioinformatics Analysis and in vitro Studies[J]. Current Cancer Drug Targets,2022,22(2):142-152.
APA Shi, Wei,Qi, Lu,You, Xiong Bin,Chen, Yu Chi,Xu, Yu Lian,Yu, Wei Bang,Huang, Mu Yang,Zhao, Hong,&Lu, Jin Jian.(2022).Identification of AHSA1 as a Potential Therapeutic Target for Breast Cancer: Bioinformatics Analysis and in vitro Studies.Current Cancer Drug Targets,22(2),142-152.
MLA Shi, Wei,et al."Identification of AHSA1 as a Potential Therapeutic Target for Breast Cancer: Bioinformatics Analysis and in vitro Studies".Current Cancer Drug Targets 22.2(2022):142-152.
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