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Activation of AMPK/miR-181b Axis Alleviates Endothelial Dysfunction and Vascular Inflammation in Diabetic Mice
Cheng, Chak-Kwong; Shang, Wenbin; Liu, Jian; Cheang, Wai San; Wang, Yu; Xiang, Li; Lau, Chi-Wai; Luo, Jiang-Yun; Ng, Chi-Fai; Huang, Yu; Wang, Li
2022-06
Source PublicationAntioxidants
ISSN2076-3921
Volume11Issue:6
Abstract

Hyperglycemia in diabetes mellitus impairs endothelial function and disrupts microRNA (miRNA) profiles in vasculature, increasing the risk of diabetes-associated complications, including coronary artery disease, diabetic retinopathy, and diabetic nephropathy. miR-181b was previously reported to be an anti-inflammatory mediator in vasculature against atherosclerosis. The current study aimed to investigate whether miR-181b ameliorates diabetes-associated endothelial dysfunction, and to identify potential molecular mechanisms and upstream inducer of miR-181b. We found that miR-181b level was decreased in renal arteries of diabetic patients and in advanced glycation end products (AGEs)-treated renal arteries of non-diabetic patients. Transfection of miR-181b mimics improved endothelium-dependent vasodilation in aortas of high fat diet (HFD)/streptozotocin (STZ)-induced diabetic mice, accompanied by suppression of superoxide overproduction and vascular inflammation markers. AMPK activator-induced AMPK activation upregulated miR-181b level in human umbilical vein endothelial cells (HUVECs). Chronic exercise, potentially through increased blood flow, activated AMPK/miR-181b axis in aortas of diabetic mice. Exposure to laminar shear stress upregulated miR-181b expression in HUVECs. Overall, our findings highlight a critical role of AMPK/miR-181b axis and extend the benefits of chronic exercise in counteracting diabetes-associated endothelial dysfunction.

KeywordMicrorna Diabetes Mellitus Endothelial Dysfunction Ros Vascular Inflammation Exercise Shear Stress
DOI10.3390/antiox11061137
URLView the original
Indexed BySCIE
WOS Research AreaBiochemistry & Molecular Biology ; Pharmacology & Pharmacy ; Food Science & Technology
WOS SubjectBiochemistry & Molecular Biology ; Chemistry, Medicinal ; Food Science & Technology
WOS IDWOS:000817602500001
Scopus ID2-s2.0-85131571786
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Cited Times [WOS]:1   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Corresponding AuthorHuang, Yu; Wang, Li
Recommended Citation
GB/T 7714
Cheng, Chak-Kwong,Shang, Wenbin,Liu, Jian,et al. Activation of AMPK/miR-181b Axis Alleviates Endothelial Dysfunction and Vascular Inflammation in Diabetic Mice[J]. Antioxidants,2022,11(6).
APA Cheng, Chak-Kwong,Shang, Wenbin,Liu, Jian,Cheang, Wai San,Wang, Yu,Xiang, Li,Lau, Chi-Wai,Luo, Jiang-Yun,Ng, Chi-Fai,Huang, Yu,&Wang, Li.(2022).Activation of AMPK/miR-181b Axis Alleviates Endothelial Dysfunction and Vascular Inflammation in Diabetic Mice.Antioxidants,11(6).
MLA Cheng, Chak-Kwong,et al."Activation of AMPK/miR-181b Axis Alleviates Endothelial Dysfunction and Vascular Inflammation in Diabetic Mice".Antioxidants 11.6(2022).
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