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Nuciferine relaxes rat mesenteric arteries through endothelium-dependent and independent mechanisms
Xinfeng Wang1,2; Wai San Cheang3; Haixia Yang1,4; Lei Xiao1; Baochang Lai1; Meiqian Zhang1; Jiahua Ni1; Zhenyu Luo1; Zihui Zhang1; Yu Huang3; Nanping Wang1,5
2015-12
Source PublicationBRITISH JOURNAL OF PHARMACOLOGY
ISSN0007-1188
Volume172Issue:23Pages:5609-5618
Abstract
BACKGROUND AND PURPOSE
Nuciferine, a constituent of lotus leaf, is an aromatic ring-containing alkaloid, with antioxidative properties. We hypothesize
nuciferine might affect vascular reactivity. This study aimed at determining the effects of nuciferine on vasomotor tone and
the underlying mechanism
EXPERIMENTAL APPROACH
Nuciferine-induced relaxations in rings of rat main mesenteric arteries were measured by wire myographs. Endothelial NOS
(eNOS) was determined by immunoblotting. Intracellular NO production in HUVECs and Ca
2+
level in both HUVECs and
vascular smooth muscle cells (VSMCs) from rat mesenteric arteries were assessed by fluorescence imaging.
KEY RESULTS
Nuciferine induced relaxations in arterial segments pre-contracted by KCl or phenylephrine. Nuciferine-elicited arterial
relaxations were reduced by removal of endothelium or by pretreatment with the eNOS inhibitor L-NAME or the NO-sensitive
guanylyl cyclase inhibitor ODQ. In HUVECs, the phosphorylation of eNOS at Ser
1177
and increase in cytosolic NO level induced
by nuciferine were mediated by extracellular Ca
2+
influx. Under endothelium-free conditions, nuciferine attenuated
CaCl
2
-induced contraction in Ca
2+
-free depolarizing medium. In the absence of extracellular calcium, nuciferine relieved the
vasoconstriction induced by phenylephrine and the addition of CaCl
2
. Nuciferine also suppressed Ca
2+
influx in Ca
2+
-free
K
+
-containing solution in VSMCs.
CONCLUSIONS AND IMPLICATIONS
Nuciferine has a vasorelaxant effect via both endothelium-dependent and -independent mechanisms. These results suggest
that nuciferine may have a therapeutic effect on vascular diseases associated with aberrant vasoconstriction.
 
DOIhttps://doi.org/10.1111/bph.13021
Indexed BySCIE
Language英語English
WOS Research AreaPharmacology & Pharmacy
WOS SubjectPharmacology & Pharmacy
WOS IDWOS:000366392500017
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Cited Times [WOS]:19   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Corresponding AuthorNanping Wang
Affiliation1.Cardiovascular Research Center
2.Department of Physiology and Pathophysiology, School of Medicine
3.Institute of Vascular Medicine, School of Biomedical Sciences, Chinese University of Hong Kong, Hong Kong, China
4.School of Public Health, Xi’an Jiaotong University, Xi’an, China
5.Institute of Cardiovascular Sciences, Peking University Health Science Center, Beijing, China
Recommended Citation
GB/T 7714
Xinfeng Wang,Wai San Cheang,Haixia Yang,et al. Nuciferine relaxes rat mesenteric arteries through endothelium-dependent and independent mechanisms[J]. BRITISH JOURNAL OF PHARMACOLOGY,2015,172(23):5609-5618.
APA Xinfeng Wang,Wai San Cheang,Haixia Yang,Lei Xiao,Baochang Lai,Meiqian Zhang,Jiahua Ni,Zhenyu Luo,Zihui Zhang,Yu Huang,&Nanping Wang.(2015).Nuciferine relaxes rat mesenteric arteries through endothelium-dependent and independent mechanisms.BRITISH JOURNAL OF PHARMACOLOGY,172(23),5609-5618.
MLA Xinfeng Wang,et al."Nuciferine relaxes rat mesenteric arteries through endothelium-dependent and independent mechanisms".BRITISH JOURNAL OF PHARMACOLOGY 172.23(2015):5609-5618.
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