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Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy
Li, M.; Huang, Y.; Song, S.; Shuang, S.; Wang, R.; Dong, C.
2021-08-01
Source PublicationDyes and Pigments
ISSN01437208, 18733743
Volume256,Pages:109727-109727
Abstract

The change in the level of ONOO− is usually indicative of an abnormality in bodily function. Therefore, there is a need to develop highly reliable ONOO− assays to determine its role in the biological environment. Thus, a long- wavelength anthracycline-based fluorescent probe (LAP) with a new reaction site was presented to detect per- oxynitrite (ONOO− ) by one step synthesize between 6-hydroxy-1-tetralone and salicylaldehyde. LAP responded to ONOO− about 8-fold fluorescence changes at 628 nm and the detection limit was 3.7 nM. The ONOO− sensing of LAP with the disruption of the conjugated skeleton was highly specific and could identify ONOO− by naked eyes. LAP was specifically localized to the mitochondria and co-localization results of LAP and MitoTrancker Green showed that the merged fluorescent images were observed with high Pearson’s co-localization coefficient 0.92 and 0.90 in SMMC-7721 and RAW264.7 cells, respectively. As a specific and sensitive fluorescence probe, LAP with mitochondria-targetable moiety was ability to detect exogenous ONOO− in SMMC-7721 and RAW264.7 cells.

KeywordLong-wavelength Anthracycline Fluorescent Probe Peroxynitrite Co-localization
URLView the original
Language英語English
The Source to ArticlePB_Publication
PUB ID58872
Document TypeJournal article
CollectionUniversity of Macau
Recommended Citation
GB/T 7714
Li, M.,Huang, Y.,Song, S.,et al. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy[J]. Dyes and Pigments,2021,256,:109727-109727.
APA Li, M.,Huang, Y.,Song, S.,Shuang, S.,Wang, R.,&Dong, C..(2021).Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy.Dyes and Pigments,256,,109727-109727.
MLA Li, M.,et al."Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy".Dyes and Pigments 256,(2021):109727-109727.
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