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Real-time visualization of efficient Nitroxyl (HNO) releasing using fluorescence technique
Guo, Qingwei1; Wu, Yangyang1; Zhang, Lei2; Qin, Yao2; Bao, Jiaolin3,4; Feng, Yuhong1; Liu, Yuanyuan1; Zhou, Yang1
2022-10-15
Source PublicationSensors and Actuators B: Chemical
ISSN0925-4005
Volume369
Abstract

Direct visualization of Nitroxyl (HNO) release is currently not feasible for all chemical and biological systems, limiting the understanding of its biological and pharmaceutical activities. To address this issue, N-hydroxydansylamide was developed as the first fluorescence turn-off HNO donor that efficiently releases HNO with a fluorescence turn-on probe. Under physiological conditions, the HNO release was observed with a half-life of 19.86 min. The decomposition of N-hydroxydansylamide yielded a fluorescent dansyl-based sulfinate species. Specifically, the concentration of HNO released was successfully monitored based on the fluorescence signal with a limit of detection of ~ 0.55 µM. Particularly, HNO releasing can be sensed without any depletion of the HNO source. Finally, cellular imaging was also achieved to demonstrate the HNO release derived from N-hydroxydansylamide. Therefore, HNO release profiles in terms of kinetics, dose, and the site can be monitored through real-time fluorometric analysis or imaging-based studies, providing a novel platform to explore HNO biology.

KeywordCell Imaging Fluorescence Hno Donors Nitroxyl (Hno) Probe
DOI10.1016/j.snb.2022.132309
URLView the original
Indexed BySCIE
Language英語English
WOS Research AreaChemistry ; Electrochemistry ; Instruments & Instrumentation
WOS SubjectChemistry, Analytical ; Electrochemistry ; Instruments & Instrumentation
WOS IDWOS:000828711800001
Scopus ID2-s2.0-85133580478
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Cited Times [WOS]:0   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
Corresponding AuthorLiu, Yuanyuan; Zhou, Yang
Affiliation1.Key Laboratory of Advanced Materials of Tropical Island Resources of Ministry of Education, School of Chemical Engineering and Technology, Hainan University, Haikou, Hainan, 570228, China
2.College of Animal Science and Technology, Hainan University, Haikou, 570228, China
3.Key Laboratory of Tropical Biological Resources of Ministry of Education, School of Pharmaceutical Sciences, Hainan University, Haikou, 570228, China
4.State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, 999078, China
Recommended Citation
GB/T 7714
Guo, Qingwei,Wu, Yangyang,Zhang, Lei,et al. Real-time visualization of efficient Nitroxyl (HNO) releasing using fluorescence technique[J]. Sensors and Actuators B: Chemical,2022,369.
APA Guo, Qingwei,Wu, Yangyang,Zhang, Lei,Qin, Yao,Bao, Jiaolin,Feng, Yuhong,Liu, Yuanyuan,&Zhou, Yang.(2022).Real-time visualization of efficient Nitroxyl (HNO) releasing using fluorescence technique.Sensors and Actuators B: Chemical,369.
MLA Guo, Qingwei,et al."Real-time visualization of efficient Nitroxyl (HNO) releasing using fluorescence technique".Sensors and Actuators B: Chemical 369(2022).
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