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Loss of Nobox prevents ovarian differentiation from juvenile ovaries in zebrafish
Qin, Mingming1; Xie, Qingping1,2; Wu, Kun1,3; Zhou, Xianqing4; Ge, Wei1
2022-06-13
Source PublicationBiology of reproduction
Volume106Issue:6Pages:1254-1266
Abstract

As a species without master sex-determining genes, zebrafish displays high plasticity in sex differentiation, making it an excellent model for studying the regulatory mechanisms underlying gonadal differentiation and gametogenesis. Despite being a gonochorist, zebrafish is a juvenile hermaphrodite that undergoes a special phase of juvenile ovary before further differentiation into functional testis and ovary. The mechanisms underlying juvenile ovary formation and subsequent gonadal differentiation remain largely unknown. In this study, we explored the role of Nobox/nobox (new born ovary homeobox protein), another oocyte-specific transcription factor in females, in early zebrafish gonadogenesis using CRISPR/Cas9 technology. As in mammals, nobox is specifically expressed in zebrafish gonads with a dimorphic pattern at juvenile stage. In contrast to the mutant of figla (factor in the germline alpha, another oocyte-specific transcription factor), the nobox mutants showed formation of typical perinucleolar (PN) follicles at primary growth (PG) stage in juvenile gonads, suggesting occurrence of follicle assembly from cystic oocytes (chromatin nucleolar stage, CN). These follicles, however, failed to develop further to form functional ovaries, resulting in all-male phenotype. Despite its expression in adult testis, the loss of nobox did not seem to affect testis development, spermatogenesis and male spawning. In summary, our results indicate an important role for Nobox in zebrafish ovarian differentiation and early folliculogenesis.

KeywordCrispr/cas9 Gonadal Differentiation Nobox Sex Reversal Zebrafish
DOI10.1093/biolre/ioac036
URLView the original
Indexed BySCIE
Language英語English
WOS Research AreaReproductive Biology
WOS SubjectReproductive Biology
WOS IDWOS:000771154600001
Scopus ID2-s2.0-85132452486
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Document TypeJournal article
CollectionFaculty of Health Sciences
Corresponding AuthorGe, Wei
Affiliation1.Department of Biomedical Sciences and Centre of Reproduction, Development and Aging (CRDA), Faculty of Health Sciences, University of Macau, Taipa, Macau 999078, China
2.Institute of Hydrobiology, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China
3.State Key Laboratory for Biocontrol, Guangdong Provincial Key Laboratory of Marine Resources and Coastal Engineering, School of Marine Sciences, Sun Yat-sen University, Guangzhou, 510275, China
4.Department of Toxicology and Hygienic Chemistry, School of Public Health, Capital Medical University, 100069, China
First Author AffilicationCentre of Reproduction, Development and Aging
Corresponding Author AffilicationCentre of Reproduction, Development and Aging
Recommended Citation
GB/T 7714
Qin, Mingming,Xie, Qingping,Wu, Kun,et al. Loss of Nobox prevents ovarian differentiation from juvenile ovaries in zebrafish[J]. Biology of reproduction,2022,106(6):1254-1266.
APA Qin, Mingming,Xie, Qingping,Wu, Kun,Zhou, Xianqing,&Ge, Wei.(2022).Loss of Nobox prevents ovarian differentiation from juvenile ovaries in zebrafish.Biology of reproduction,106(6),1254-1266.
MLA Qin, Mingming,et al."Loss of Nobox prevents ovarian differentiation from juvenile ovaries in zebrafish".Biology of reproduction 106.6(2022):1254-1266.
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