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Maternal Prkce expression in mature oocytes is critical for the first cleavage facilitating maternal-to-zygotic transition in mouse early embryos
Zhang, Shaoqing1; Gong, Xiuli1; Zhou, Yiye1,2,3; Ma, Qingwen1,3; Cai, Qin1,3; Yang, Guanheng1,3; Guo, Xinbing1,3; Chen, Yanwen1,3; Xu, Miao1,3; Zhu, Yiwen1,3; Zeng, Yitao1,3; Zeng, Fanyi1,2,3,4
2022-06-01
Source PublicationCell Proliferation
ISSN0960-7722
Volume55Issue:6
Abstract

Objectives: Early embryo development is dependent on the regulation of maternal messages stored in the oocytes during the maternal-to-zygote transition. Previous studies reported variability of oocyte competence among different inbred mouse strains. The present study aimed to identify the maternal transcripts responsible for early embryonic development by comparing transcriptomes from oocytes of high- or low- competence mouse strains. Materials and Methods: In vitro fertilization embryos from oocytes of different mouse strains were subject to analysis using microarrays, RNA sequencing, real-time quantitative PCR (RT-qPCR) analysis, Western blotting, and immunofluorescence. One candidate gene, Prkce, was analysed using Prkce knockout mice, followed by a cRNA rescue experiment. Results: The fertilization and 2-cell rate were significantly higher for FVB/NJ (85.1% and 82.0%) and DBA/2J (79.6% and 76.7%) inbred mouse strains than those for the MRL/lpr (39.9% and 35.8%) and 129S3 (35.9% and 36.6%) strains. Thirty-nine differentially expressed genes (DEGs) were noted, of which nine were further verified by RT-qPCR. Prkce knockout mice showed a reduced 2-cell rate (Prkce 80.1% vs. Prkce 32.4%) that could be rescued by Prkce cRNA injection (2-cell rate reached 76.7%). Global transcriptional analysis revealed 143 DEGs in the knockout mice, which were largely composed of genes functioning in cell cycle regulation. Conclusions: The transcription level of maternal messages such as Prkce in mature oocytes is associated with different 2-cell rates in select inbred mouse strains. Prkce transcript levels could serve as a potential biomarker to characterize high-quality mature oocytes.

DOI10.1111/cpr.13231
URLView the original
Indexed BySCIE
Language英語English
WOS Research AreaCell Biology
WOS SubjectCell Biology
WOS IDWOS:000796867100001
Scopus ID2-s2.0-85130219752
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Document TypeJournal article
CollectionUniversity of Macau
Corresponding AuthorZeng, Fanyi
Affiliation1.School of Life Sciences and Biotechnology & Shanghai Children's Hospital, Shanghai Jiao Tong University, Shanghai, China
2.Department of Histo-Embryology, Genetics and Developmental Biology, Shanghai Jiao Tong University School of Medicine, Shanghai, China
3.NHC Key Laboratory of Medical Embryogenesis and Developmental Molecular Biology & Shanghai Key Laboratory of Embryo and Reproduction Engineering, Shanghai, China
4.School of Pharmacy, Macau University of Science and Technonlogy, Taipa, Macao
Corresponding Author AffilicationUniversity of Macau
Recommended Citation
GB/T 7714
Zhang, Shaoqing,Gong, Xiuli,Zhou, Yiye,et al. Maternal Prkce expression in mature oocytes is critical for the first cleavage facilitating maternal-to-zygotic transition in mouse early embryos[J]. Cell Proliferation,2022,55(6).
APA Zhang, Shaoqing,Gong, Xiuli,Zhou, Yiye,Ma, Qingwen,Cai, Qin,Yang, Guanheng,Guo, Xinbing,Chen, Yanwen,Xu, Miao,Zhu, Yiwen,Zeng, Yitao,&Zeng, Fanyi.(2022).Maternal Prkce expression in mature oocytes is critical for the first cleavage facilitating maternal-to-zygotic transition in mouse early embryos.Cell Proliferation,55(6).
MLA Zhang, Shaoqing,et al."Maternal Prkce expression in mature oocytes is critical for the first cleavage facilitating maternal-to-zygotic transition in mouse early embryos".Cell Proliferation 55.6(2022).
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