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Intravital lipid droplet labeling and imaging reveals the phenotypes and functions of individual macrophages in vivo
Li, Yue1; Du, Yuwei2; Xu, Zhengqing2; He, Yuan3; Yao, Ran1; Jiang, Huiran2; Ju, Wen2; Qiao, Jianlin2; Xu, Kailin2; Liu, Tzu Ming4; Zeng, Lingyu2
2022-05-01
Source PublicationJournal of lipid research
Volume63Issue:5
Abstract

Macrophages play pivotal roles in the maintenance of tissue homeostasis. However, the reactivation of macrophages toward proinflammatory states correlates with a plethora of inflammatory diseases, including atherosclerosis, obesity, neurodegeneration, and bone marrow (BM) failure syndromes. The lack of methods to reveal macrophage phenotype and function in vivo impedes the translational research of these diseases. Here, we found that proinflammatory macrophages accumulate intracellular lipid droplets (LDs) relative to resting or noninflammatory macrophages both in vitro and in vivo, indicating that LD accumulation serves as a structural biomarker for macrophage phenotyping. To realize the staining and imaging of macrophage LDs in vivo, we developed a fluorescent fatty acid analog-loaded poly(lactic-co-glycolic acid) nanoparticle to label macrophages in mice with high efficiency and specificity. Using these novel nanoparticles, we achieved in situ functional identification of single macrophages in BM, liver, lung, and adipose tissues under conditions of acute or chronic inflammation. Moreover, with this intravital imaging platform, we further realized in vivo phenotyping of individual macrophages in the calvarial BM of mice under systemic inflammation. In conclusion, we established an efficient in vivo LD labeling and imaging system for single macrophage phenotyping, which will aid in the development of diagnostics and therapeutic monitoring. Moreover, this method also provides new avenues for the study of lipid trafficking and dynamics in vivo.

KeywordBiomarker Bone Marrow Fatty Acid Analog Inflammation In vivo Imaging Lipid Droplet Lipid Trafficking Macrophage Nanoparticle Delivery Systemic Inflammation
DOI10.1016/j.jlr.2022.100207
URLView the original
Indexed BySCIE
Language英語English
WOS Research AreaBiochemistry & Molecular Biology
WOS SubjectBiochemistry & Molecular Biology
WOS IDWOS:000832827100002
Scopus ID2-s2.0-85130862707
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Cited Times [WOS]:1   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionFaculty of Health Sciences
Corresponding AuthorLiu, Tzu Ming; Zeng, Lingyu
Affiliation1.School of Medical Technology, Xuzhou Medical University, Xuzhou, China
2.Blood Diseases Institute, Xuzhou Medical University, Xuzhou, Jiangsu, China; Key Laboratory of Bone Marrow Stem Cell, Xuzhou, Jiangsu, China; Department of Hematology, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, Jiangsu, China
3.School of Pharmacy, Xuzhou Medical University, Xuzhou, China
4.Faculty of Health Sciences, University of Macau, Taipa, China
Corresponding Author AffilicationFaculty of Health Sciences
Recommended Citation
GB/T 7714
Li, Yue,Du, Yuwei,Xu, Zhengqing,et al. Intravital lipid droplet labeling and imaging reveals the phenotypes and functions of individual macrophages in vivo[J]. Journal of lipid research,2022,63(5).
APA Li, Yue,Du, Yuwei,Xu, Zhengqing,He, Yuan,Yao, Ran,Jiang, Huiran,Ju, Wen,Qiao, Jianlin,Xu, Kailin,Liu, Tzu Ming,&Zeng, Lingyu.(2022).Intravital lipid droplet labeling and imaging reveals the phenotypes and functions of individual macrophages in vivo.Journal of lipid research,63(5).
MLA Li, Yue,et al."Intravital lipid droplet labeling and imaging reveals the phenotypes and functions of individual macrophages in vivo".Journal of lipid research 63.5(2022).
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