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Structural basis of SARS-CoV-2 and its variants binding to intermediate horseshoe bat ACE2
Tang, Lingfeng1,2; Zhang, Di1,2; Han, Pu1; Kang, Xinrui1,3; Zheng, Anqi1,3; Xu, Zepeng1,2; Zhao, Xin1; Wang, Vivien Ya Fan2; Qi, Jianxun1,3; Wang, Qihui1,3; Liu, Kefang1; Gao, George F.1,2
2022
Source PublicationInternational Journal of Biological Sciences
ISSN1449-2288
Volume18Issue:11Pages:4658-4668
Abstract

Coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has caused a global pandemic. Intermediate horseshoe bats (Rhinolophus affinis) are hosts of RaTG13, one of the second most phylogenetically related viruses to SARS-CoV-2. We report the binding between intermediate horseshoe bat ACE2 (bACE2-Ra) and SARS-CoV-2 receptor-binding domain (RBD), supporting the pseudotyped SARS-CoV-2 viral infection. A 3.3 Å resolution crystal structure of the bACE2-Ra/SARS-CoV-2 RBD complex was determined. The interaction networks of Patch 1 showed differences in R34 and E35 of bACE2-Ra compared to hACE2 and big-eared horseshoe bat ACE2 (bACE2-Rm). The E35K substitution, existing in other species, significantly enhanced the binding affinity owing to its electrostatic attraction with E484 of SARS-CoV-2 RBD. Furthermore, bACE2-Ra showed extensive support for the SARS-CoV-2 variants. These results broaden our knowledge of the ACE2/RBD interaction mechanism and emphasize the importance of continued surveillance of intermediate horseshoe bats to prevent spillover risk.

KeywordBace2-ra Intermediate Horseshoe Bats Sars-cov-2 Sars-cov-2 Variants Structure
DOI10.7150/ijbs.73640
URLView the original
Indexed BySCIE
Language英語English
WOS Research AreaBiochemistry & Molecular Biology ; Life Sciences & Biomedicine - Other Topics
WOS SubjectBiochemistry & Molecular Biology ; Biology
WOS IDWOS:000828439300017
Scopus ID2-s2.0-85134244851
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Cited Times [WOS]:0   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
Affiliation1.CAS Key Laboratory of Pathogen Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China
2.Faculty of Health Sciences, University of Macau, 999078, China
3.University of Chinese Academy of Sciences, Beijing, 100049, China
First Author AffilicationFaculty of Health Sciences
Recommended Citation
GB/T 7714
Tang, Lingfeng,Zhang, Di,Han, Pu,et al. Structural basis of SARS-CoV-2 and its variants binding to intermediate horseshoe bat ACE2[J]. International Journal of Biological Sciences,2022,18(11):4658-4668.
APA Tang, Lingfeng,Zhang, Di,Han, Pu,Kang, Xinrui,Zheng, Anqi,Xu, Zepeng,Zhao, Xin,Wang, Vivien Ya Fan,Qi, Jianxun,Wang, Qihui,Liu, Kefang,&Gao, George F..(2022).Structural basis of SARS-CoV-2 and its variants binding to intermediate horseshoe bat ACE2.International Journal of Biological Sciences,18(11),4658-4668.
MLA Tang, Lingfeng,et al."Structural basis of SARS-CoV-2 and its variants binding to intermediate horseshoe bat ACE2".International Journal of Biological Sciences 18.11(2022):4658-4668.
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