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Ratiometric ATP Detection on Gliding Microtubules Based on Bioorthogonal Fluorescence Ligation
Feng, G.; Zhang, B.; Wang, S.; Ding, Z.; Li, G.; Zhang, X.
2019-09-04
Source PublicationSensors and Actuators B: Chemical
ISSN0925-4005
Pages127090-1-127090-7
AbstractAn ingenious microtubule functionalization strategy based on the novel bioorthogonal conjugation is developed to construct fluorescent ratiometric probes for ATP sensing. By single excitation, newly synthesized probe RT-1 exhibited green emission (485 nm) from the bioorthogonal fluorescence conjugation part and orange emission (584 nm) from the ATP sensing part. The fluorescence intensity ratio (I584/I485) displayed good linear response in ATP ranges of 0 - 2.5 mM (y=0.9282x+0.2398, R2 = 0.9705, LOD = 0.0354 mM) and 2.0 - 10.0 mM (y=2.7153x-3.6234, R2 = 0.9911, LOD = 0.0121 mM). Moreover, a paclitaxel derivate probe RT-2 was developed to functionalize in vitro polymerized microtubule for in situ ATP detection. This simple and convenient strategy is anticipated to stimulate more microtubule related applications in both in vitro and intracellular studies.
KeywordBioorthogonal Fluorescence Inverse Electron Demand Diels–Alder Reaction ATP Microtubule Ratiometric Sensing
URLView the original
Language英語English
The Source to ArticlePB_Publication
PUB ID47102
Document TypeJournal article
CollectionDEPARTMENT OF BIOMEDICAL SCIENCES
Corresponding AuthorZhang, X.
Recommended Citation
GB/T 7714
Feng, G.,Zhang, B.,Wang, S.,et al. Ratiometric ATP Detection on Gliding Microtubules Based on Bioorthogonal Fluorescence Ligation[J]. Sensors and Actuators B: Chemical,2019:127090-1-127090-7.
APA Feng, G.,Zhang, B.,Wang, S.,Ding, Z.,Li, G.,&Zhang, X..(2019).Ratiometric ATP Detection on Gliding Microtubules Based on Bioorthogonal Fluorescence Ligation.Sensors and Actuators B: Chemical,127090-1-127090-7.
MLA Feng, G.,et al."Ratiometric ATP Detection on Gliding Microtubules Based on Bioorthogonal Fluorescence Ligation".Sensors and Actuators B: Chemical (2019):127090-1-127090-7.
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