UM  > Institute of Chinese Medical Sciences
Affiliated with RCfalse
Status已發表Published
Citri Reticulatae Pericarpium extract and flavonoids reduce inflammation in RAW 264.7 macrophages by inactivation of MAPK and NF-κB pathways
Zhang, Xutao; Zhou, Yan; Cheong, Meng Sam; Khan, Haroon; Ruan, C.C.; Fu, Manqin; Xiao, Jianbo; Cheang, Wai San
2022-07
Source PublicationFood Frontiers
ISSN2643-8429
Abstract

Citri reticulatae Pericarpium (CRP) is widely consumed as a food and herbal medicine in various countries and has many biological activities, including anti-inflammatory effects, but the molecular mechanism is not completely known. This study aims to investigate whether CRP extract and its major flavonoids hesperidin (Hsd) and hesperetin (Hst) can reduce inflammation in macrophages and the underlying mechanism. The chemical composition of CRP was detected by ultra-performance liquid chromatography‒mass spectrometry (UPLC‒MS) analysis. The inflammatory cell model was induced by stimulating RAW 264.7 cells with lipopolysaccharide (LPS, 1 μg/ml). Levels of nitric oxide (NO) and cytokines were determined by Griess assay and enzyme-linked immunosorbent assay kits, respectively. Protein expressions were examined by western blotting. CRP extract dose-dependently suppressed LPS-induced production of NO, interleukin-6 (IL-6), and tumor necrosis factor-α. Elevated expressions of inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2) and phosphorylation of c-Jun NH2-terminal kinase, extracellular signal-regulated kinase, p38, and p65 in LPS-stimulated cells were prevented by CRP extract. Hsd and Hst are flavonoids present in CRP, as confirmed by UPLC‒MS analysis. Both Hsd and Hst ameliorated LPS-triggered inflammation to reduce NO release, IL-6 generation, and protein expressions of iNOS and COX-2. These results reveal that CRP extract possesses anti-inflammatory activity by inactivating the mitogen-activated protein kinase and nuclear factor kappa-light chain-enhancer of activated B cells pathways, where Hsd and Hst are bioactive constituents, implying the potential application of CRP as dietary supplements against inflammatory diseases.

KeywordAnti-inflammation Citri Reticulatae Pericarpium Lipopolysaccharide Macrophages Mapk Nf-κb
DOI10.1002/fft2.169
Language英語English
The Source to ArticlePB_Publication
Scopus ID2-s2.0-85135132089
Fulltext Access
Citation statistics
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Corresponding AuthorXiao, Jianbo; Cheang, Wai San
Recommended Citation
GB/T 7714
Zhang, Xutao,Zhou, Yan,Cheong, Meng Sam,et al. Citri Reticulatae Pericarpium extract and flavonoids reduce inflammation in RAW 264.7 macrophages by inactivation of MAPK and NF-κB pathways[J]. Food Frontiers,2022.
APA Zhang, Xutao,Zhou, Yan,Cheong, Meng Sam,Khan, Haroon,Ruan, C.C.,Fu, Manqin,Xiao, Jianbo,&Cheang, Wai San.(2022).Citri Reticulatae Pericarpium extract and flavonoids reduce inflammation in RAW 264.7 macrophages by inactivation of MAPK and NF-κB pathways.Food Frontiers.
MLA Zhang, Xutao,et al."Citri Reticulatae Pericarpium extract and flavonoids reduce inflammation in RAW 264.7 macrophages by inactivation of MAPK and NF-κB pathways".Food Frontiers (2022).
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Zhang, Xutao]'s Articles
[Zhou, Yan]'s Articles
[Cheong, Meng Sam]'s Articles
Baidu academic
Similar articles in Baidu academic
[Zhang, Xutao]'s Articles
[Zhou, Yan]'s Articles
[Cheong, Meng Sam]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Zhang, Xutao]'s Articles
[Zhou, Yan]'s Articles
[Cheong, Meng Sam]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.