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LampPort: a handheld digital microfluidic device for loop-mediated isothermal amplification (LAMP)
Liang Wan1; Jie Gao1; Tianlan Chen1; Cheng Dong1; Haoran Li1; Yan-Zi Wen2; Zhao-Rong Lun2; Yanwei Jia1; Pui-In Mak1; Rui P. Martins1,3
2019-03-01
Source PublicationBiomedical Microdevices
ISSN1387-2176
Volume21Issue:1
Abstract

A major goal in the development of point-of-care (POC) devices is to build them as portable to provide a rapid and effective determination for disease pathogens. In nucleic acid testing, an optical detection system used to monitor the product of nucleic acid amplification has always been a bulky accessory. In this work, we developed a handheld, automatic and detection system-free thermal digital microfluidic (DMF) device for DNA detection by loop-mediated isothermal amplification (LAMP). Droplet manipulation and real-time temperature control systems were integrated into a handheld device. The control software could be installed into any tablet and communicate with the device via Bluetooth. In the experimentation, we loaded 2-μl samples with an electrowetting force into sandwich-structured DMF chips, thereby considerably reducing reagent consumptions. After an on-chip LAMP reaction, we added a highly concentrated SYBR Green I droplet and mixed it with a reaction droplet to enable product detection with the naked eye. This step prevented aerosol contamination by avoiding the exposure of the reaction droplet to the air. Using a blood parasite Trypanosoma brucei as a model system, this system showed similar results as a commercial thermal cycler and could detect 40 copies per reaction of the DNA target. This low-cost, compact device removed the bulky optical system for DNA detection, thus enabling it to be well suited for POC testing.

KeywordDigital Microfluidic System Handheld Device Loop-mediated Isothermal Amplification (Lamp) Naked-eye Visualisation
DOI10.1007/s10544-018-0354-9
URLView the original
Indexed BySCIE
WOS Research AreaEngineering ; Science & Technology - Other Topics
WOS SubjectEngineering, Biomedical ; Nanoscience & Nanotechnology
WOS IDWOS:000455092800002
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Citation statistics
Cited Times [WOS]:28   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionFaculty of Health Sciences
INSTITUTE OF MICROELECTRONICS
Corresponding AuthorYanwei Jia
Affiliation1.University of Macau
2.Sun Yat-Sen University
3.Instituto Superior Técnico
First Author AffilicationUniversity of Macau
Corresponding Author AffilicationUniversity of Macau
Recommended Citation
GB/T 7714
Liang Wan,Jie Gao,Tianlan Chen,et al. LampPort: a handheld digital microfluidic device for loop-mediated isothermal amplification (LAMP)[J]. Biomedical Microdevices,2019,21(1).
APA Liang Wan,Jie Gao,Tianlan Chen,Cheng Dong,Haoran Li,Yan-Zi Wen,Zhao-Rong Lun,Yanwei Jia,Pui-In Mak,&Rui P. Martins.(2019).LampPort: a handheld digital microfluidic device for loop-mediated isothermal amplification (LAMP).Biomedical Microdevices,21(1).
MLA Liang Wan,et al."LampPort: a handheld digital microfluidic device for loop-mediated isothermal amplification (LAMP)".Biomedical Microdevices 21.1(2019).
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