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Spatiotemporal control of engineered bacteria to express interferon-γ by focused ultrasound for tumor immunotherapy
Yuhao Chen1; Meng Du1; Zhen Yuan1,2; Zhiyi Chen1; Fei Yan1
2022-08-02
Source PublicationNature Communication
ISSN2041-1723
Volume13Issue:1Pages:1-15
Abstract

Bacteria-based tumor therapy has recently attracted wide attentions due to its unique capability in targeting tumors and preferentially colonizing the core area of the tumor. Various therapeutic genes are also harbored into these engineering bacteria to enhance their anti-tumor efficacy. However, it is difficult to spatiotemporally control the expression of these inserted genes in the tumor site. Here, we engineer an ultrasound-responsive bacterium (URB) which can induce the expression of exogenous genes in an ultrasound-controllable manner. Owing to the advantage of ultrasound in tissue penetration, an acoustic remote control of bacterial gene expression can be realized by designing a temperature-actuated genetic switch. Cytokine interferon-γ (IFN-γ), an important immune regulatory molecule that plays a significant role in tumor immunotherapy, is used to test the system. Our results show that brief hyperthermia induced by focused ultrasound promotes the expression of IFN-γ gene, improving anti-tumor efficacy of URB in vitro and in vivo. Our study provides an alternative strategy for bacteria-mediated tumor immunotherapy.

Keyword医学影象学, 光学工程, 肿瘤治疗学
Indexed BySCIE
Language英語English
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS IDWOS:000836703600026
The Source to Articlehttps://www.nature.com/articles/s41467-022-31932-x.pdf
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Document TypeJournal article
CollectionDEPARTMENT OF PUBLIC HEALTH AND MEDICINAL ADMINISTRATION
Faculty of Health Sciences
INSTITUTE OF COLLABORATIVE INNOVATION
Corresponding AuthorZhiyi Chen; Fei Yan
Affiliation1.Faculty of Health Sciences, University of Macau
2.Centre for Cognitive and Brain Sciences, University of Macau
First Author AffilicationFaculty of Health Sciences
Corresponding Author AffilicationFaculty of Health Sciences
Recommended Citation
GB/T 7714
Yuhao Chen,Meng Du,Zhen Yuan,et al. Spatiotemporal control of engineered bacteria to express interferon-γ by focused ultrasound for tumor immunotherapy[J]. Nature Communication,2022,13(1):1-15.
APA Yuhao Chen,Meng Du,Zhen Yuan,Zhiyi Chen,&Fei Yan.(2022).Spatiotemporal control of engineered bacteria to express interferon-γ by focused ultrasound for tumor immunotherapy.Nature Communication,13(1),1-15.
MLA Yuhao Chen,et al."Spatiotemporal control of engineered bacteria to express interferon-γ by focused ultrasound for tumor immunotherapy".Nature Communication 13.1(2022):1-15.
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