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Accelerating precision anti-cancer therapy by time-lapse and label-free 3D tumor slice culture platform
Xing, F.; Huang, S.; Liu, Y.-C.; Lyu, X.; Su, S. M.; Chan, U. I.; Wu, P.-C.; Yan, Y.; Ai, N.; Li, J.; Zhao, M.; Rajendran, B.; Liu, J.; Shao, F.; Sun, H.; Luo, G.; Zhu, W.; Miao, K.; Zhao, Q.; Luo, KQ.; Ge, W.; Xu, X.; Wang, G.; Liu, T.-M.; Deng, C.-X.
2021-09-13
Source PublicationTheranostics
ISSN1838-7640
Pages9415-9430
Abstract

The feasibility and reliability of personalized medicine for cancer treatment is largely hampered by inefficient and oversimplified evaluation of anti-cancer drug response. Here, we report the generation and characterization of three-dimensional tumor slice culture (3D-TSC) platform for rapid anti-cancer drug discovery. Histological analysis of biomarkers and RNA sequence analysis demonstrate that 3D-TSCs accurately preserve cell repertoire and immune components of its original tumor. Using FRET based biosensor sensor C3 (C3) as an apoptotic reporter, we performed time-lapse high-throughput 166 anti-cancer drug screening on five cancer cell lines, which shortlists high efficacy drugs to screen human and murine 3D-TSCs and nominate effective candidates within 7 days after the surgery, a necessary speed for precision drug discovery. Further application of human and murine 3D-TSCs for immune check point blockade assays was successfully achieved with antibodies to PD-1 and/or PD-L1. Finally, revealed by label-free multi-photon fluorescence imaging, 3D-TSCs exhibit lipofuscin autofluorescence features for the time-course monitoring of drug response and efficacy. Overall, our time-course evaluation techniques on 3D-TSC platform demonstrate strong potential for predicting the efficacy of anti-cancer drugs and providing efficient and rapid drug screening platform for personalized chemotherapy and immunotherapy.

KeywordTumor Organoid Lipofuscin Two-photon Fluorescence Microscopy
Language英語English
The Source to ArticlePB_Publication
Document TypeJournal article
CollectionDEPARTMENT OF BIOMEDICAL SCIENCES
Corresponding AuthorWang, G.; Liu, T.-M.; Deng, C.-X.
Recommended Citation
GB/T 7714
Xing, F.,Huang, S.,Liu, Y.-C.,et al. Accelerating precision anti-cancer therapy by time-lapse and label-free 3D tumor slice culture platform[J]. Theranostics,2021:9415-9430.
APA Xing, F..,Huang, S..,Liu, Y.-C..,Lyu, X..,Su, S. M..,Chan, U. I..,Wu, P.-C..,Yan, Y..,Ai, N..,Li, J..,Zhao, M..,Rajendran, B..,Liu, J..,Shao, F..,Sun, H..,Luo, G..,Zhu, W..,Miao, K..,Zhao, Q..,...&Deng, C.-X..(2021).Accelerating precision anti-cancer therapy by time-lapse and label-free 3D tumor slice culture platform.Theranostics,9415-9430.
MLA Xing, F.,et al."Accelerating precision anti-cancer therapy by time-lapse and label-free 3D tumor slice culture platform".Theranostics (2021):9415-9430.
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