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Metformin protects RPE cells from oxidative damage by enhancing autophagy through activation of AMPK pathway
Zhao, X.; Zheng, W.
2020-12-01
Conference NameMetformin protects RPE cells from oxidative damage by enhancing autophagy through activation of AMPK pathway
Source Publication2020 Academic Annual Meeting of Chronic Disease Prevention and Management Professional Committee of Chinese Society of Integrative Medicine and Geriatrics Professional Committee of World Federation of
Pages82-83
Conference Date2020-12-01
Conference PlaceN/A
Abstract

Age-related macular degeneration (AMD) is a leading cause of blindness with very limited effective treatments. Although the pathogenesis of this disease being complex and not fully understood, the oxidative damage caused by excessive reactive oxygen species (ROS) in the retinal pigment epithelium (RPE), has been considered as a major cause. Autophagy is essential for the degradation of cellular components damaged by ROS and its dysregulation has been implicated in AMD pathogenesis. Therefore, strategies aiming to boost autophagy could be effective in protecting RPE cells from oxidative damage. Metformin is the first-line anti-type 2 diabetes drug and has been reported to stimulate autophagy in many tissues. We therefore hypothesized that metformin may be able to protect RPE cells against H2O2-induced oxidative damage by autophagy activation. In the present study, we found that metformin attenuated H2O2-induced cell viability loss, apoptosis, elevated ROS levels as well as the collapse of the mitochondria membrane potential in D407 cells. Autophagy was stimulated by metformin, and inhibition of autophagy by 3-Methyladenine and chloroquine or knockdown of Beclin1 and LC3B blocked the protective effects of metformin. In addition, we showed that metformin could activate the AMPK pathway, whereas both pharmacological and genetic inhibition of AMPK blocked the autophagy-stimulating and protective effects of metformin. Metformin conferred a similar protection against H2O2-induced oxidative damage in primary cultured human RPE cells. Taken together, these results demonstrate that metformin could protect RPE cells from H2O2-induced oxidative damage by stimulating autophagy via the activation of AMPK pathway, supporting its potential use in the prevention and treatment of AMD.

KeywordAmd Metformin Autophagy Ampk Pathway Rpe Cells
Language英語English
The Source to ArticlePB_Publication
Document TypeConference paper
CollectionDEPARTMENT OF PHARMACEUTICAL SCIENCES
Faculty of Health Sciences
Corresponding AuthorZheng, W.
Recommended Citation
GB/T 7714
Zhao, X.,Zheng, W.. Metformin protects RPE cells from oxidative damage by enhancing autophagy through activation of AMPK pathway[C],2020:82-83.
APA Zhao, X.,&Zheng, W..(2020).Metformin protects RPE cells from oxidative damage by enhancing autophagy through activation of AMPK pathway.2020 Academic Annual Meeting of Chronic Disease Prevention and Management Professional Committee of Chinese Society of Integrative Medicine and Geriatrics Professional Committee of World Federation of,82-83.
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