UM  > Faculty of Health Sciences  > DEPARTMENT OF PHARMACEUTICAL SCIENCES
Status已發表Published
Protective effect of metformin against hydrogen peroxide induced oxidative damage in RPE cells by enhancing autophagy through activation of AMPK pathway Abstract
Zheng, W.
2020-05-19
Source Publication4th Symposium on Biomedical Sciences for Students, Postdoctoral Fellows and Research Assistants 2020
AbstractAge-related macular degeneration (AMD) is a leading cause of blindness with very limited effective treatment. Although the pathogenesis of this disease is complex and not fully understood, the oxidative damage caused by excessive ROS in RPE, has been considered as a major cause. Autophagy is essential for the degradation of cellular components damaged by ROS and its dysregulation has been implicated in AMD pathogenesis. Therefore, strategies aiming to boost autophagy could be effective in protecting RPE cells from oxidative damage. Metformin is the first-line anti-type 2 diabetes drug and has been reported to stimulate autophagy in many tissues. We therefore hypothesized that metformin may be able to protect RPE cells against H2O2-induced oxidative damage by autophagy activation. In the present study, we found that metformin attenuated H2O2-induced cell viability loss, apoptosis, elevated ROS levels as well as the collapse of the mitochondria membrane potential in D407 cells. Autophagy was stimulated by metformin, and inhibition of autophagy by 3-Methyladenine and chloroquine or knockdown of Beclin1 and LC3B blocked the protective effects of metformin. In addition, we showed that metformin could activate the AMPK pathway, whereas both pharmacological and genetic inhibition of AMPK blocked the autophagy-stimulating and protective effects of metformin. Metformin conferred a similar protection against H2O2-induced oxidative damage in primary cultured human RPE cells. Taken together, these results demonstrate that metformin could protect RPE cells from H2O2-induced oxidative damage by stimulating autophagy via the activation of AMPK
KeywordAMD hydrogen peroxide-induce oxidative damage AMPK Pathway
Language英語English
The Source to ArticlePB_Publication
PUB ID51490
Document TypeConference paper
CollectionDEPARTMENT OF PHARMACEUTICAL SCIENCES
Corresponding AuthorZheng, W.
Recommended Citation
GB/T 7714
Zheng, W.. Protective effect of metformin against hydrogen peroxide induced oxidative damage in RPE cells by enhancing autophagy through activation of AMPK pathway Abstract[C],2020.
APA Zheng, W..(2020).Protective effect of metformin against hydrogen peroxide induced oxidative damage in RPE cells by enhancing autophagy through activation of AMPK pathway Abstract.4th Symposium on Biomedical Sciences for Students, Postdoctoral Fellows and Research Assistants 2020.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Zheng, W.]'s Articles
Baidu academic
Similar articles in Baidu academic
[Zheng, W.]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Zheng, W.]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.