UM  > Faculty of Health Sciences  > DEPARTMENT OF PHARMACEUTICAL SCIENCES
Residential Collegetrue
Status已發表Published
Artemisinin protected human SH-SY5Y and hippocampal neurons from hydrogen peroxide (H2O2)-induced oxidative damage through the activation of the AMPK pathway
Zheng, W.
2018-09-01
Conference NameAiwan Asia-Pacific Aging Protection Association
Source PublicationAiwan Asia-Pacific Aging Protection Association
Conference Date2018-9
Conference Place-
Abstract

Abstract: Oxidative stress is one of the main causes of neurodegenerative diseases such as Alzheimer's disease (AD). Our previous studies have found the neuroprotective effect of artemisinin, an anti-malaria Chinese medicine, however, the antioxidative effect of artemisinin and its potential mechanism remain to be elucidated. In the present study, the protective effect and the underlying mechanism of artemisinin against injury of hydrogen peroxide (H2O2) in SH-SY5Y and hippocampal neurons were studied. Our results show that artemisinin, at clinically relevant concentrations, protected SH-SY5Y and hippocampal neuronal cells from H2O2-induced cell death in a concentration-dependent manner. Further studies showed that artemisinin significantly reduced cell death caused by H2O2 by restoring nuclear morphology, abnormal changes in intracellular ROS, activation of caspase-3, lactate dehydrogenase release and mitochondrial membrane potential. Hoechst staining and flow cytometry showed that artemisinin significantly reduced the apoptosis of SH-SY5Y cells exposed to H2O2. Western blot analysis showed that artemisinin stimulated the phosphorylation and activation of AMP-activated protein kinase (AMPK) in SH-SY5Y cells in a time and concentration-dependent manner, whereas the application of AMPK inhibitor Compound C or decrease in expression of AMPKα with shRNA specific for AMPKα blocked the protective effect of artemisinin. Similar results were obtained in primary cultured hippocampal neurons. Taken together, these results indicate that artemisinin can protect neuronal cells from oxidative damage, at least in part through the activation of AMPK.

KeywordArtemisinin Alzheimer's Disease Amp-activated Protein Kinase (Ampk)
Language英語English
The Source to ArticlePB_Publication
Document TypeConference paper
CollectionDEPARTMENT OF PHARMACEUTICAL SCIENCES
Faculty of Health Sciences
Corresponding AuthorZheng, W.
Recommended Citation
GB/T 7714
Zheng, W.. Artemisinin protected human SH-SY5Y and hippocampal neurons from hydrogen peroxide (H2O2)-induced oxidative damage through the activation of the AMPK pathway[C],2018.
APA Zheng, W..(2018).Artemisinin protected human SH-SY5Y and hippocampal neurons from hydrogen peroxide (H2O2)-induced oxidative damage through the activation of the AMPK pathway.Aiwan Asia-Pacific Aging Protection Association.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Zheng, W.]'s Articles
Baidu academic
Similar articles in Baidu academic
[Zheng, W.]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Zheng, W.]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.