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Single-cell multiomics sequencing reveals the reprogramming defects in embryos generated by round spermatid injection
Wang, Jing1; Zhou, Cai1; Gao, Shuai2; Song, Xiuling1; Yang, Xinyan1; Fan, Jiaqi3; Ren, Shaofang1; Ma, Linzi1; Zhao, Jiexiang1; Cui, Manman1; Song, Ke1; Wang, Mei1; Li, Chaohui1; Zheng, Yi1; Luo, Fang1; Miao, Kai4; Bai, Xiaochun1; Hutchins, Andrew P.5; Li, Lin3; Chang, Gang6; Zhao, Xiao Yang1,7,8
2022-08-12
Source PublicationScience Advances
ISSN2375-2548
Volume8Issue:32
Abstract

Round spermatid injection (ROSI) technique holds great promise for clinical treatment of a proportion of infertile men. However, the compromised developmental potential of ROSI embryos largely limits the clinical application, and the mechanisms are not fully understood. Here, we describe the transcriptome, chromatin accessibility, and DNA methylation landscapes of mouse ROSI embryos derived from early-stage round spermatids using a single-cell multiomics sequencing approach. By interrogating these data, we identify the reprogramming defects in ROSI embryos at the pronuclear stages, which are mainly associated with the misexpression of a cohort of minor zygotic genome activation genes. We screen a small compound, A366, that can significantly increase the developmental potential of ROSI embryos, in which A366 can partially overcome the reprogramming defects by amending the epigenetic and transcriptomic states. Collectively, our study uncovers the reprogramming defects in ROSI embryos for understanding the mechanisms underlying compromised developmental potential and offers an avenue for ROSI technique optimization.

DOI10.1126/sciadv.abm3976
URLView the original
Indexed BySCIE
Language英語English
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS IDWOS:000841491100006
Scopus ID2-s2.0-85135934750
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Cited Times [WOS]:0   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionDEPARTMENT OF BIOMEDICAL SCIENCES
Affiliation1.State Key Laboratory of Organ Failure Research, Department of Developmental Biology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, Guangdong, 510515, China
2.Key Laboratory of Animal Genetics, Breeding and Reproduction of the MARA, National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing, 100193, China
3.Guangdong Provincial Key Laboratory of Proteomics, Department of Pathophysiology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, 510515, China
4.Center for Precision Medicine Research and Training, Faculty of Health Sciences, University of Macau, Macao
5.Department of Biology, Southern University of Science and Technology, Shenzhen, Guangdong, 518060, China
6.Department of Biochemistry and Molecular Biology, Shenzhen University Health Science Center, Shenzhen, Guangdong, 518060, China
7.Guangdong Key Laboratory of Construction and Detection in Tissue Engineering, Southern Medical University, Guangzhou, Guangdong, 510515, China
8.Guangzhou Regenerative Medicine and Health Guangdong Laboratory (GRMH-GDL), Guangzhou, Guangdong, 510700, China
Recommended Citation
GB/T 7714
Wang, Jing,Zhou, Cai,Gao, Shuai,et al. Single-cell multiomics sequencing reveals the reprogramming defects in embryos generated by round spermatid injection[J]. Science Advances,2022,8(32).
APA Wang, Jing.,Zhou, Cai.,Gao, Shuai.,Song, Xiuling.,Yang, Xinyan.,Fan, Jiaqi.,Ren, Shaofang.,Ma, Linzi.,Zhao, Jiexiang.,Cui, Manman.,Song, Ke.,Wang, Mei.,Li, Chaohui.,Zheng, Yi.,Luo, Fang.,Miao, Kai.,Bai, Xiaochun.,Hutchins, Andrew P..,Li, Lin.,...&Zhao, Xiao Yang.(2022).Single-cell multiomics sequencing reveals the reprogramming defects in embryos generated by round spermatid injection.Science Advances,8(32).
MLA Wang, Jing,et al."Single-cell multiomics sequencing reveals the reprogramming defects in embryos generated by round spermatid injection".Science Advances 8.32(2022).
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