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A tRNA-derived fragment from Chinese yew suppresses ovarian cancer growth via targeting TRPA1
Cao, Kai Yue1; Yan, Tong Meng1; Zhang, Ji Zhou2; Chan, Ting Fung2; Li, Jie3; Li, Chong3; Lai-Han Leung, Elaine1; Gao, Jin4; Zhang, Bao Xian5; Jiang, Zhi Hong1
2022-03-08
Source PublicationMolecular Therapy - Nucleic Acids
Volume27Pages:718-732
AbstractDrug discovery from plants usually focuses on small molecules rather than such biological macromolecules as RNAs. Although plant transfer RNA (tRNA)-derived fragment (tRF) has been associated with the developmental and defense mechanisms in plants, its regulatory role in mammals remains unclear. By employing a novel reverse small interfering RNA (siRNA) screening strategy, we show that a tRF mimic (antisense derived from the 5′ end of tRNA of Chinese yew) exhibits comparable anti-cancer activity with that of taxol on ovarian cancer A2780 cells, with a 16-fold lower dosage than that of taxol. A dual-luciferase reporter assay revealed that tRF-T11 directly targets the 3′ UTR of oncogene TRPA1 mRNA. Furthermore, an Argonaute-RNA immunoprecipitation (AGO-RIP) assay demonstrated that tRF-T11 can interact with AGO2 to suppress TRPA1 via an RNAi pathway. This study uncovers a new role of plant-derived tRFs in regulating endogenous genes. This holds great promise for exploiting novel RNA drugs derived from nature and sheds light on the discovery of unknown molecular targets of therapeutics.
Keywordovarian cancer Taxus chinensis tRF tRNA TRPA1
DOI10.1016/j.omtn.2021.12.037
URLView the original
Language英語English
Scopus ID2-s2.0-85122806797
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Cited Times [WOS]:5   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
Affiliation1.State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Taipa, Avenida Wai Long, China
2.State Key Laboratory of Agrobiotechnology, School of Life Sciences, The Chinese University of Hong Kong, Shatin, China
3.Medical Research Institute, College of Pharmaceutical Sciences, Southwest University, Chongqing, 400715, China
4.Increasepharm (Hengqin) Institute Co., Ltd, Zhuhai, 519031, China
5.Increasepharm (HK) Limited, China
First Author AffilicationUniversity of Macau
Recommended Citation
GB/T 7714
Cao, Kai Yue,Yan, Tong Meng,Zhang, Ji Zhou,et al. A tRNA-derived fragment from Chinese yew suppresses ovarian cancer growth via targeting TRPA1[J]. Molecular Therapy - Nucleic Acids,2022,27:718-732.
APA Cao, Kai Yue,Yan, Tong Meng,Zhang, Ji Zhou,Chan, Ting Fung,Li, Jie,Li, Chong,Lai-Han Leung, Elaine,Gao, Jin,Zhang, Bao Xian,&Jiang, Zhi Hong.(2022).A tRNA-derived fragment from Chinese yew suppresses ovarian cancer growth via targeting TRPA1.Molecular Therapy - Nucleic Acids,27,718-732.
MLA Cao, Kai Yue,et al."A tRNA-derived fragment from Chinese yew suppresses ovarian cancer growth via targeting TRPA1".Molecular Therapy - Nucleic Acids 27(2022):718-732.
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