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Apoptosis-Sensing Xenograft Zebrafish Tumor Model for Anticancer Evaluation of Redox-Responsive Cross-Linked Pluronic Micelles
Tao, Jinsong1,5; Wei, Zhengjie1; Cheng, Yaxin1; Xu, Meng1; Li, Qiuxia1; Lee, Simon Ming Yuen1; Ge, Wei2; Luo, Kathy Qian2,3; Wang, Xueqing4; Zheng, Ying1,2,3
2022-09-07
Source PublicationACS Applied Materials and Interfaces
ISSN1944-8244
Volume14Issue:35Pages:39775-39786
Abstract

A suitable animal model for preclinical screening and evaluation in vivo could vastly increase the efficiency and success rate of nanomedicine development. Compared with rodents, the transparency of the zebrafish model offers unique advantages of real-time and high-resolution imaging of the whole body and cellular levels in vivo. In this research, we established an apoptosis-sensing xenograft zebrafish tumor model to evaluate the anti-cancer effects of redox-responsive cross-linked Pluronic polymeric micelles (CPPMs) visually and accurately. First, doxorubicin (Dox)-loaded CPPMs were fabricated and characterized with glutathione (GSH)-responsive drug release. Then, the B16F10 xenograft zebrafish tumor model was established to mimic the tumor microenvironment with angiogenesis and high GSH generation for redox-responsive tumor-targeting evaluation in vivo. The high GSH generation was first verified in the xenograft zebrafish tumor model. Compared with ordinary Pluronic polymeric micelles, Dox CPPMs had a much higher accumulation in zebrafish tumor sites. Finally, the apoptosis-sensing B16F10-C3 xenograft zebrafish tumor model was established for visual, rapid, effective, and noninvasive assessment of anti-cancer effects at the cellular level in vivo. The Dox CPPMs significantly inhibited the proliferation of cancer cells and induced apoptosis in the B16F10-C3 xenograft zebrafish tumor model. Therefore, the redox-responsive cross-linked Pluronic micelles showed effective anti-cancer therapy in the xenograft zebrafish tumor model. This xenograft zebrafish tumor model is available for rapid screening and assessment of anti-cancer effects in preclinical studies.

KeywordAnti-cancer Disulfide Cross-linking Pluronic Polymeric Micelles Redox Response Tumor Microenvironment Xenograft Zebrafish Tumor Model
DOI10.1021/acsami.2c09005
URLView the original
Indexed BySCIE
Language英語English
WOS Research AreaScience & Technology - Other Topics ; Materials Science
WOS SubjectNanoscience & Nanotechnology ; Materials Science, Multidisciplinary
WOS IDWOS:000848406800001
Scopus ID2-s2.0-85137625792
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Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Faculty of Health Sciences
Affiliation1.State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, 999078, Macao
2.Faculty of Health Sciences, University of Macau, 999078, Macao
3.MOE Frontier Science Centre for Precision Oncology, University of Macau, 999078, Macao
4.Beijing Key Laboratory of Molecular Pharmaceutics and New Drug Delivery System, School of Pharmaceutical Sciences, Peking University, Beijing, 100191, China
5.Institute of Applied Physics and Materials Engineering, University of Macau, 999078, Macao
First Author AffilicationInstitute of Chinese Medical Sciences;  INSTITUTE OF APPLIED PHYSICS AND MATERIALS ENGINEERING
Recommended Citation
GB/T 7714
Tao, Jinsong,Wei, Zhengjie,Cheng, Yaxin,et al. Apoptosis-Sensing Xenograft Zebrafish Tumor Model for Anticancer Evaluation of Redox-Responsive Cross-Linked Pluronic Micelles[J]. ACS Applied Materials and Interfaces,2022,14(35):39775-39786.
APA Tao, Jinsong,Wei, Zhengjie,Cheng, Yaxin,Xu, Meng,Li, Qiuxia,Lee, Simon Ming Yuen,Ge, Wei,Luo, Kathy Qian,Wang, Xueqing,&Zheng, Ying.(2022).Apoptosis-Sensing Xenograft Zebrafish Tumor Model for Anticancer Evaluation of Redox-Responsive Cross-Linked Pluronic Micelles.ACS Applied Materials and Interfaces,14(35),39775-39786.
MLA Tao, Jinsong,et al."Apoptosis-Sensing Xenograft Zebrafish Tumor Model for Anticancer Evaluation of Redox-Responsive Cross-Linked Pluronic Micelles".ACS Applied Materials and Interfaces 14.35(2022):39775-39786.
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