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Nardoguaianone L Isolated from Nardostachys jatamansi Improved the Effect of Gemcitabine Chemotherapy via Regulating AGE Signaling Pathway in SW1990 Cells
Zheng, Yi Dan1,2; Ma, Li Mei1,3; Lu, Jin Jian4; Chai, Tian1; Naghavi, Mohammad Reza5; Ma, Jun Yi2; Sang, Chun Yan1; Yang, Jun Li1
2022-10-01
Source PublicationMolecules
ISSN1420-3049
Volume27Issue:20
Abstract

Pancreatic cancer is the seventh leading cause of cancer-related death worldwide and is known as “the king of cancers”. Currently, gemcitabine (GEM) as the clinical drug of choice for chemotherapy of advanced pancreatic cancer has poor drug sensitivity and ineffective chemotherapy. Nardoguaianone L (G-6) is a novel guaiane-type sesquiterpenoid isolated from Nardostachys jatamansi DC., and it exhibits anti-tumor activity. Based on the newly discovered G-6 with anti-pancreatic cancer activity in our laboratory, this paper aimed to evaluate the potential value of the combination of G-6 and GEM in SW1990 cells, including cell viability, cell apoptosis, colony assay and tandem mass tags (TMT) marker-based proteomic technology. These results showed that G-6 combined with GEM significantly inhibited cell viability, and the effect was more obvious than that with single drug. In addition, the use of TMT marker-based proteomic technology demonstrated that the AGE-RAGE signaling pathway was activated after medication-combination. Furthermore, reactive oxygen species (ROS) and mitochondrial membrane potential (MMP) assays were used to validate the proteomic results. Finally, apoptosis was detected by flow cytometry. In conclusion, G-6 combined with GEM induced an increase in ROS level and a decrease in MMP in SW1990 cells through the AGE-RAGE signaling pathway, ultimately leading to apoptosis. G-6 improved the effect of GEM chemotherapy and may be used as a potential combination therapy for pancreatic cancer.

KeywordAge-rage Signaling Pathway Gemcitabine Pancreatic Cancer Proteomic Sw1990 Cells
DOI10.3390/molecules27206849
URLView the original
Indexed BySCIE
Language英語English
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS SubjectBiochemistry & Molecular Biology ; Chemistry, Multidisciplinary
WOS IDWOS:000873385500001
Scopus ID2-s2.0-85140889200
Fulltext Access
FWCI0.94354546
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Cited Times [WOS]:1   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Affiliation1.CAS Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou, 730000, China
2.College of Life Science, Northwest Normal University, Lanzhou, 730070, China
3.University of Chinese Academy of Sciences, Chinese Academy of Sciences, Beijing, 100049, China
4.State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao
5.Agricultural Natural Resources College, University of Tehran, Karaj, 3158777871, Iran
Recommended Citation
GB/T 7714
Zheng, Yi Dan,Ma, Li Mei,Lu, Jin Jian,et al. Nardoguaianone L Isolated from Nardostachys jatamansi Improved the Effect of Gemcitabine Chemotherapy via Regulating AGE Signaling Pathway in SW1990 Cells[J]. Molecules,2022,27(20).
APA Zheng, Yi Dan,Ma, Li Mei,Lu, Jin Jian,Chai, Tian,Naghavi, Mohammad Reza,Ma, Jun Yi,Sang, Chun Yan,&Yang, Jun Li.(2022).Nardoguaianone L Isolated from Nardostachys jatamansi Improved the Effect of Gemcitabine Chemotherapy via Regulating AGE Signaling Pathway in SW1990 Cells.Molecules,27(20).
MLA Zheng, Yi Dan,et al."Nardoguaianone L Isolated from Nardostachys jatamansi Improved the Effect of Gemcitabine Chemotherapy via Regulating AGE Signaling Pathway in SW1990 Cells".Molecules 27.20(2022).
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