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Double-Sided Sapphire Optrodes with Conductive Shielding Layers to Reduce Optogenetic Stimulation Artifacts
Shen, Junyu1; Xu, Yanyan1; Xiao, Zhengwen1; Liu, Yuebo1; Liu, Honghui1; Wang, Fengge1; Yan, Chaokun1; Wang, Liyang2; Chen, Changhao2; Wu, Zhisheng1,3; Liu, Yang1,3; Mak, Peng Un4; Vai, Mang I.2,4; Pun, Sio Hang2; Lei, Tim C.5; Zhang, Baijun1,3
Source PublicationMicromachines

Optrodes, which are single shaft neural probes integrated with microelectrodes and optical light sources, offer a remarkable opportunity to simultaneously record and modulate neural activities using light within an animal’s brain; however, a common problem with optrodes is that stimulation artifacts can be observed in the neural recordings of microelectrodes when the light source on the optrode is activated. These stimulation artifacts are undesirable contaminants, and they cause interpretation complexity when analyzing the recorded neural activities. In this paper, we tried to mitigate the effects of the stimulation artifacts by developing a low-noise, double-sided optrode integrated with multiple Electromagnetic Shielding (EMS) layers. The LED and microelectrodes were constructed separately on the top epitaxial and bottom substrate layers, and EMS layers were used to separate the microelectrodes and LED to reduce signal cross-talks. Compared with conventional single-sided designs, in which the LED and microelectrodes are constructed on the same side, our results indicate that double-sided optrodes can significantly reduce the presence of stimulation artifacts. In addition, the presence of stimulation artifacts can further be reduced by decreasing the voltage difference and increasing the rise/fall time of the driving LED pulsed voltage. With all these strategies, the presence of stimulation artifacts was significantly reduced by ~76%. As well as stimulation suppression, the sapphire substrate also provided strong mechanical stiffness and support to the optrodes, as well as improved electronic stability, thus making the double-sided sapphire optrodes highly suitable for optogenetic neuroscience research on animal models.

KeywordDouble-sided Optrode Electromagnetic Shielding Layers Microelectrodes Sapphire Substrate Stimulation Artifacts
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Indexed BySCIE
WOS Research AreaChemistry ; Science & Technology - Other Topics ; Instruments & Instrumentation ; Physics
WOS SubjectChemistry, Analytical ; Nanoscience & Nanotechnology ; Instruments & Instrumentation ; Physics, Applied
WOS IDWOS:000881262500001
Scopus ID2-s2.0-85141769025
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Document TypeJournal article
Affiliation1.School of Electronics and Information Technology, Sun Yat-sen University, Guangzhou, 510006, China
2.State Key Laboratory of Analog and Mixed-Signal VLSI, Institute of Microelectronics, University of Macau, 999078, Macao
3.State Key Laboratory of Optoelectronic Materials and Technologies, Sun Yat-sen University, Guangzhou, 510275, China
4.Department of Electrical and Computer Engineering, Faculty of Science and Technology, University of Macau, 999078, Macao
5.Department of Electrical Engineering, University of Colorado, Denver, 80204, United States
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GB/T 7714
Shen, Junyu,Xu, Yanyan,Xiao, Zhengwen,et al. Double-Sided Sapphire Optrodes with Conductive Shielding Layers to Reduce Optogenetic Stimulation Artifacts[J]. Micromachines,2022,13(11).
APA Shen, Junyu,Xu, Yanyan,Xiao, Zhengwen,Liu, Yuebo,Liu, Honghui,Wang, Fengge,Yan, Chaokun,Wang, Liyang,Chen, Changhao,Wu, Zhisheng,Liu, Yang,Mak, Peng Un,Vai, Mang I.,Pun, Sio Hang,Lei, Tim C.,&Zhang, Baijun.(2022).Double-Sided Sapphire Optrodes with Conductive Shielding Layers to Reduce Optogenetic Stimulation Artifacts.Micromachines,13(11).
MLA Shen, Junyu,et al."Double-Sided Sapphire Optrodes with Conductive Shielding Layers to Reduce Optogenetic Stimulation Artifacts".Micromachines 13.11(2022).
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