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An assay for systematically quantifying the vestibulo-ocular reflex to assess vestibular function in Zebrafish Larvae
Sun, Peng1,2,3; Zhang, Yingla4; Zhao, Feng5; Wu, Jian-Ping3,6; Pun, Sio Hang1; Peng, Cheng3; Du, Meide3; Vai, Mang I.1,2; Liu, Dong4,5; Chen, Fangyi3
2018-08-21
Source PublicationFrontiers in Cellular Neuroscience
ISSN1662-5102
Volume12
Abstract

Zebrafish (Danio rerio) larvae are widely used to study otic functions because they possess all five typical vertebrate senses including hearing and balance. Powerful genetic tools and the transparent body of the embryo and larva also make zebrafish a unique vertebrate model to study otic development. Due to its small larval size and moisture requirement during experiments, accurately acquiring the vestibulo-ocular reflex (VOR) of zebrafish larva is challenging. In this report, a new VOR testing device has been developed for quantifying linear VOR (LVOR) in zebrafish larva, evoked by the head motion about the earth horizontal axis. The system has a newly designed larva-shaped chamber, by which live fish can be steadily held without anesthesia, and the system is more compact and easier to use than its predecessors. To demonstrate the efficacy of the system, the LVORs in wild-type (WT), dlx3b and dlx4b morphant zebrafish larvae were measured and the results showed that LVOR amplitudes were consistent with the morphological changes of otoliths induced by morpholino oligonucleotides (MO). Our study represents an important advance to obtain VOR and predict the vestibular conditions in zebrafish.

KeywordGene Knock Down Otolith Vestibular Function Vestibulo-ocular Reflex Zebrafish Larva
DOI10.3389/fncel.2018.00257
URLView the original
Indexed BySCIE
Language英語English
WOS Research AreaNeurosciences ; Neurology
WOS IDWOS:000442263900001
Fulltext Access
Citation statistics
Cited Times [WOS]:5   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionDEPARTMENT OF ELECTRICAL AND COMPUTER ENGINEERING
THE STATE KEY LABORATORY OF ANALOG AND MIXED-SIGNAL VLSI (UNIVERSITY OF MACAU)
Corresponding AuthorVai, Mang I.; Liu, Dong; Chen, Fangyi
Affiliation1.Univ Macau, State Key Lab Analog & MixedSignal VLSI, Taipa, Macao, Peoples R China
2.Univ Macau, Fac Sci & Technol, Dept Elect & Comp Engn, Taipa, Macao, Peoples R China
3.Southern Univ Sci & Technol, Dept Biomed Engn, Shenzhen, Peoples R China
4.Peking Univ, Sch Life Sci, Beijing, Peoples R China
5.Southern Univ Sci & Technol, Dept Biol, Shenzhen, Peoples R China
6.Southern Univ Sci & Technol, SUSTech Acad Adv Interdisciplinary Studies, Shenzhen, Peoples R China
First Author AffilicationUniversity of Macau
Corresponding Author AffilicationUniversity of Macau
Recommended Citation
GB/T 7714
Sun, Peng,Zhang, Yingla,Zhao, Feng,et al. An assay for systematically quantifying the vestibulo-ocular reflex to assess vestibular function in Zebrafish Larvae[J]. Frontiers in Cellular Neuroscience,2018,12.
APA Sun, Peng,Zhang, Yingla,Zhao, Feng,Wu, Jian-Ping,Pun, Sio Hang,Peng, Cheng,Du, Meide,Vai, Mang I.,Liu, Dong,&Chen, Fangyi.(2018).An assay for systematically quantifying the vestibulo-ocular reflex to assess vestibular function in Zebrafish Larvae.Frontiers in Cellular Neuroscience,12.
MLA Sun, Peng,et al."An assay for systematically quantifying the vestibulo-ocular reflex to assess vestibular function in Zebrafish Larvae".Frontiers in Cellular Neuroscience 12(2018).
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