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Sailuotong Prevents Hydrogen Peroxide (H2O2)-Induced Injury in EA.hy926 Cells
Seto, Sai Wang1; Chang, Dennis1; Ko, Wai Man1; Zhou, Xian1; Kiat, Hosen2,3,4; Bensoussan, Alan1; Lee, Simon M. Y.5; Hoi, Maggie P. M.5; Steiner, Genevieve Z.1; Liu, Jianxun1,6

Sailuotong (SLT) is a standardised three-herb formulation consisting of Panax ginseng, Ginkgo biloba, and Crocus sativus designed for the management of vascular dementia. While the latest clinical trials have demonstrated beneficial effects of SLT in vascular dementia, the underlying cellular mechanisms have not been fully explored. The aim of this study was to assess the ability and mechanisms of SLT to act against hydrogen peroxide (H2O2)-induced oxidative damage in cultured human vascular endothelial cells (EAhy926). SLT (1-50 mu g/mL) significantly suppressed the H2O2-induced cell death and abolished the H2O2-induced reactive oxygen species (ROS) generation in a concentration-dependent manner. Similarly, H2O2 (0.5 mM; 24 h) caused a similar to 2-fold increase in lactate dehydrogenase (LDH) release from the EA. hy926 cells which were significantly suppressed by SLT (1-50 mu g/mL) in a concentration-dependent manner. Incubation of SLT (50 mu g/mL) increased superoxide dismutase (SOD) activity and suppressed the H2O2-enhanced Bax/Bcl-2 ratio and cleaved caspase-3 expression. In conclusion, our results suggest that SLT protects EA. hy916 cells against H2O2-mediated injury via direct reduction of intracellular ROS generation and an increase in SOD activity. These protective effects are closely associated with the inhibition of the apoptotic death cascade via the suppression of caspase-3 activation and reduction of Bax/Bcl-2 ratio, thereby indicating a potential mechanism of action for the clinical effects observed.

KeywordReactive Oxygen Species Sailuotong (Slt) Herbal Medicine Apoptosis Endothelial Dysfunction Vascular Dementia
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Indexed BySCIE
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS SubjectBiochemistry & Molecular Biology ; Chemistry, Multidisciplinary
WOS IDWOS:000393030600094
The Source to ArticleWOS
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Cited Times [WOS]:17   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Corresponding AuthorSeto, Sai Wang
Affiliation1.National Institute of Complementary Medicine (NICM), Western Sydney University, Penrith, NSW 2571, Australia
2.Faculty of Medicine, University of New South Wales, NSW 2052, Australia
3.School of Medicine, Western Sydney University, Locked Bag 1797, Penrith, NSW 2751, Australia
4.Faculty of Medicine and Health Sciences, Macquarie University, NSW 2109, Australia
5.State Key Laboratory Research in Chinese Medicine and Institute of Chinese Medical Sciences, University of Macau, Macao, China
6.Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing 100091, China
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Seto, Sai Wang,Chang, Dennis,Ko, Wai Man,et al. Sailuotong Prevents Hydrogen Peroxide (H2O2)-Induced Injury in EA.hy926 Cells[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2017,18(1).
APA Seto, Sai Wang,Chang, Dennis,Ko, Wai Man,Zhou, Xian,Kiat, Hosen,Bensoussan, Alan,Lee, Simon M. Y.,Hoi, Maggie P. M.,Steiner, Genevieve Z.,&Liu, Jianxun.(2017).Sailuotong Prevents Hydrogen Peroxide (H2O2)-Induced Injury in EA.hy926 Cells.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,18(1).
MLA Seto, Sai Wang,et al."Sailuotong Prevents Hydrogen Peroxide (H2O2)-Induced Injury in EA.hy926 Cells".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 18.1(2017).
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