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Identifying the core bacterial microbiome of hydrocarbon degradation and a shift of dominant methanogenesis pathways in the oil and aqueous phases of petroleum reservoirs of different temperatures from China
Zhou, Zhichao1; Liang, Bo2; Wang, Li-Ying2; Liu, Jin-Feng2; Mu, Bo-Zhong2; Shim, Hojae3; Gu, Ji-Dong1
2019-11-08
Source PublicationBIOGEOSCIENCES
ISSN1726-4170
Volume16Issue:1Pages:4229-4241
Abstract

Microorganisms in petroleum reservoirs play significant roles in hydrocarbon degradation, and through the terminal electron-accepting process of methanogenesis, they also contribute to microbially enhanced oil recovery (MEOR) worldwide, with great economic and environmental benefits. Here, a molecular investigation, using the 16S rRNA and mcrA gene profiles based on MiSeq sequencing and clone library construction methods, was conducted on oil and water (aqueous) phases of samples of high (82-88 degrees C), moderate (45-63 degrees C), and low temperatures (21-32 degrees C) from seven petroleum reservoirs in China. A core bacterial microbiome with a small proportion of shared operational taxonomic unit (OTU) values, but a high proportion of sequences among all reservoirs was discovered, including aerobic degraders, sulfate- and nitrate-reducing bacteria, fermentative bacteria, and sulfur-oxidizing bacteria distributed mainly in Proteobacteria, Bacteroidetes, Deferribacteres, Deinococcus-Thermus, Firmicutes, Spirochaetes, and Thermotogae. Their prevalence in the previously reported petroleum reservoirs and successive enrichment cultures suggests their common roles and functions involved in aliphatic and aromatic hydrocarbon degradation. The methanogenic process generally shifts from the dominant hydrogenotrophic pathway in the aqueous phase to the acetoclastic pathway in the oil phase in high-temperature reservoirs, but the opposite was true for low-temperature reservoirs. No difference was detected between the two phases in moderate temperature reservoirs. Physicochemical factors, including pH; temperature; phase conditions; and nitrate, Mn2+, and Mg2+ concentrations were the main factors correlated to the microbial compositional and functional profiles significantly. Linear discriminant analysis (LDA) effect size (LEfSe) analysis shows distribution differences of microbial groups towards pH, temperature, and the oil and aqueous phases. Using the software Tax4Fun for functional profiling indicated functional metabolism differences between the two phases, including amino acids, hydrocarbons in the oil phase, and carbohydrates in the aqueous phase.

DOI10.5194/bg-16-4229-2019
Indexed BySCIE
Language英語English
WOS Research AreaEnvironmental Sciences & Ecology ; Geology
WOS SubjectEcology ; Geosciences, Multidisciplinary
WOS IDWOS:000496716400002
PublisherCOPERNICUS GESELLSCHAFT MBH, BAHNHOFSALLEE 1E, GOTTINGEN 37081, GERMANY
Scopus ID2-s2.0-85075004348
Fulltext Access
FWCI0.6532749
Citation statistics
Document TypeJournal article
CollectionDEPARTMENT OF CIVIL AND ENVIRONMENTAL ENGINEERING
Corresponding AuthorMu, Bo-Zhong; Gu, Ji-Dong
Affiliation1.Laboratory of Environmental Microbiology and Toxicology, School of Biological Sciences, The University of Hong Kong, Pokfulam Road, Hong Kong, Hong Kong SAR, People’s Republic of China
2.State Key Laboratory of Bioreactor Engineering and Institute of Applied Chemistry, East China University of Science and Technology, Shanghai 200237, People’s Republic of China
3.Faculty of Science and Technology, University of Macau, Macau, People’s Republic of China
Recommended Citation
GB/T 7714
Zhou, Zhichao,Liang, Bo,Wang, Li-Ying,et al. Identifying the core bacterial microbiome of hydrocarbon degradation and a shift of dominant methanogenesis pathways in the oil and aqueous phases of petroleum reservoirs of different temperatures from China[J]. BIOGEOSCIENCES,2019,16(1):4229-4241.
APA Zhou, Zhichao,Liang, Bo,Wang, Li-Ying,Liu, Jin-Feng,Mu, Bo-Zhong,Shim, Hojae,&Gu, Ji-Dong.(2019).Identifying the core bacterial microbiome of hydrocarbon degradation and a shift of dominant methanogenesis pathways in the oil and aqueous phases of petroleum reservoirs of different temperatures from China.BIOGEOSCIENCES,16(1),4229-4241.
MLA Zhou, Zhichao,et al."Identifying the core bacterial microbiome of hydrocarbon degradation and a shift of dominant methanogenesis pathways in the oil and aqueous phases of petroleum reservoirs of different temperatures from China".BIOGEOSCIENCES 16.1(2019):4229-4241.
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